ProtParam
User-provided sequence:
10 20 30 40 50 60
MDYSNFGNSA SKKFQDDTLN RVRKEHEEAL KKLREENFSS NTSELGNKKH YRAQERMSSP
70 80 90 100 110 120
LHRLSPTGKS DDRKVKSPLD DKLRRQLREG NTRLPPPPFS SYGMPPTNRS NLDRIRRRTS
130 140 150 160 170 180
SPVRTDKFAS QNVIDDQRLE IKYLERIVYD QGTVIDNLTS RITRLESFIL NSISDRGDKN
190 200 210 220 230 240
FASLEHSRSF SGFPTNKTYG LQMGGLYEND MPYRRSSDNI NKEGAREDRS SQIHIENEST
250
EDILKILSSS FHN
References and
documentation are available.
Number of amino acids: 253
Molecular weight: 29280.46
Theoretical pI: 9.49
Total number of negatively charged residues (Asp + Glu): 37
Total number of positively charged residues (Arg + Lys): 44
Atomic composition:
Carbon C 1256
Hydrogen H 2012
Nitrogen N 390
Oxygen O 409
Sulfur S 5
Formula: C
1256H
2012N
390O
409S
5
Total number of atoms: 4072
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11920
Abs 0.1% (=1 g/l) 0.407
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 66.88
This classifies the protein as unstable.
Aliphatic index: 61.66
Grand average of hydropathicity (GRAVY): -1.143