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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MDYSNFGNSA SKKFQDDTLN RVRKEHEEAL KKLREENFSS NTSELGNKKH YRAQERMSSP

70 80 90 100 110 120
LHRLSPTGKS DDRKVKSPLD DKLRRQLREG NTRLPPPPFS SYGMPPTNRS NLDRIRRRTS

130 140 150 160 170 180
SPVRTDKFAS QNVIDDQRLE IKYLERIVYD QGTVIDNLTS RITRLESFIL NSISDRGDKN

190 200 210 220 230 240
FASLEHSRSF SGFPTNKTYG LQMGGLYEND MPYRRSSDNI NKEGAREDRS SQIHIENEST

250
EDILKILSSS FHN


References and documentation are available.

Number of amino acids: 253 Molecular weight: 29280.46 Theoretical pI: 9.49
Amino acid composition: 
Ala (A) 6 2.4% Arg (R) 27 10.7% Asn (N) 19 7.5% Asp (D) 19 7.5% Cys (C) 0 0.0% Gln (Q) 8 3.2% Glu (E) 18 7.1% Gly (G) 12 4.7% His (H) 6 2.4% Ile (I) 13 5.1% Leu (L) 21 8.3% Lys (K) 17 6.7% Met (M) 5 2.0% Phe (F) 10 4.0% Pro (P) 12 4.7% Ser (S) 33 13.0% Thr (T) 13 5.1% Trp (W) 0 0.0% Tyr (Y) 8 3.2% Val (V) 6 2.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 37 Total number of positively charged residues (Arg + Lys): 44 Atomic composition: Carbon C 1256 Hydrogen H 2012 Nitrogen N 390 Oxygen O 409 Sulfur S 5 Formula: C1256H2012N390O409S5 Total number of atoms: 4072 Extinction coefficients: This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 11920 Abs 0.1% (=1 g/l) 0.407 Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 66.88 This classifies the protein as unstable. Aliphatic index: 61.66 Grand average of hydropathicity (GRAVY): -1.143