ProtParam
User-provided sequence:
10 20 30 40 50 60
MEADDHVSLF RFPFKIPTFR GIRKGGVYLS GALYALGFWI FLDAVLYSRY SNASDVHVTF
70 80 90 100 110 120
IDWIPFLCST LGTLIVNSIE KNRLLQGALS SDGGAFGSGV GDLDSSMAWQ ARTVLFFGFA
130 140 150 160 170 180
LLAGGLSGSI VVLIIKFLVK DYNTYPTLGM GVNNVLGNVC ILLSCVVLWI AQNVEDEYSY
SLTL
References and
documentation are available.
Number of amino acids: 184
Molecular weight: 20101.29
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 14
Total number of positively charged residues (Arg + Lys): 11
Atomic composition:
Carbon C 932
Hydrogen H 1426
Nitrogen N 226
Oxygen O 257
Sulfur S 6
Formula: C
932H
1426N
226O
257S
6
Total number of atoms: 2847
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 34045
Abs 0.1% (=1 g/l) 1.694, assuming all pairs of Cys residues form cystines
Ext. coefficient 33920
Abs 0.1% (=1 g/l) 1.687, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 22.67
This classifies the protein as stable.
Aliphatic index: 113.86
Grand average of hydropathicity (GRAVY): 0.586