ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEAEPLETEQ DEWRKERMRV LDEWCEIELK GADALKMDVT REPGSPTVAV APETSLQDLY 

        70         80         90        100        110        120 
PLLIATSSSN APPSATPTDD VTDPEVQVVS RESIDNRSTF NRIIDVCLCR PVKSQMGPKA 

       130        140        150        160        170        180 
YTDKTLILKL AQIPYDHENG THWLLLSDYF NNVSRSLMTS SEYSHVTNPS RVGAHWVTVG 

       190        200        210        220        230        240 
FQSATPHTDF RGCGVLGLLQ MHTFTQRVPA NLLRAIVLLA TTEPNDFPLA VVSINITSIL 

       250        260        270        280        290        300 
LTQLKKGALD NFGNEIEGLY PFFSALHASA MCRFCSIYKS QKCTLANTQT IFSEITRQLE 

       310        320 
KSPLSLIMLL NPTNDELINT LL 

References and documentation are available.

Number of amino acids: 322

Molecular weight: 35889.95

Theoretical pI: 5.14

Amino acid composition: 
Ala (A)  21	  6.5%
Arg (R)  15	  4.7%
Asn (N)  16	  5.0%
Asp (D)  17	  5.3%
Cys (C)   7	  2.2%
Gln (Q)  12	  3.7%
Glu (E)  21	  6.5%
Gly (G)  12	  3.7%
His (H)   7	  2.2%
Ile (I)  17	  5.3%
Leu (L)  39	 12.1%
Lys (K)  12	  3.7%
Met (M)   8	  2.5%
Phe (F)  11	  3.4%
Pro (P)  20	  6.2%
Ser (S)  27	  8.4%
Thr (T)  29	  9.0%
Trp (W)   4	  1.2%
Tyr (Y)   7	  2.2%
Val (V)  20	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 27

Atomic composition:

Carbon      C	      1588
Hydrogen    H	      2523
Nitrogen    N	       425
Oxygen      O	       490
Sulfur      S	        15

Formula: C1588H2523N425O490S15
Total number of atoms: 5041

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32805
Abs 0.1% (=1 g/l)   0.914, assuming all pairs of Cys residues form cystines


Ext. coefficient    32430
Abs 0.1% (=1 g/l)   0.904, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.52
This classifies the protein as unstable.



Aliphatic index: 92.36

Grand average of hydropathicity (GRAVY): -0.152