ProtParam
User-provided sequence:
10 20 30 40 50 60
MEAEPPPSRT LPYGTSETID DDEALESKRW KRRHVVAILA LLGFANIYAM RANLSIAIVE
70 80 90 100 110 120
MTSGTERKVN GTTLHVLGDF ENWTPMTQGV VLSSFFYGYI VSQLPGGYLA YTHGAKTIFF
130 140 150 160 170 180
AGTFGTAVFT LLTPPFARMG YGMLVFARFM EGLLEGVTYP AMHVIWSRWA PPMEQTKLAT
190 200 210 220 230 240
FAFSGSYFGT VVAMPLSAYL GEHFGWPMIF WFFGALGVIW CMVWYKTVHD RPEDDPKIST
250 260 270 280 290 300
SELALLQRDA VSQNHYIVPW AQILRSKPVW AVIVAHSAQN LGFYIMLTNL PKMLKDIAGY
310 320 330 340 350 360
NVEKAGIASS LPYFLMGFQI ITGGQLCDYL RRDKHYDTLF VRKMACALGF IGQSVFLFLV
370 380 390 400 410 420
MTTSNSLLLV LFFSISIGLG GICWCGFSVN HLDLAPQYAG HLMATSNTFA TIPGIFGPLL
430 440 450 460 470
VGAIVQNGTI GEWNVIMYII ISAYLLGAAI FWKFADATLQ PWAAEHTSFV GQLE
References and
documentation are available.
Number of amino acids: 474
Molecular weight: 52631.34
Theoretical pI: 6.45
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 2450
Hydrogen H 3694
Nitrogen N 598
Oxygen O 646
Sulfur S 24
Formula: C
2450H
3694N
598O
646S
24
Total number of atoms: 7412
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 107050
Abs 0.1% (=1 g/l) 2.034, assuming all pairs of Cys residues form cystines
Ext. coefficient 106800
Abs 0.1% (=1 g/l) 2.029, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.06
This classifies the protein as stable.
Aliphatic index: 96.33
Grand average of hydropathicity (GRAVY): 0.389