ProtParam
User-provided sequence:
10 20 30 40 50 60
MEAHNQFLKT FQKERHDMKE AEKDEILLME NSRRFVMFPI KYHEIWAAYK KVEASFWTAE
70 80 90 100 110 120
EIELAKDTED FQKLTDDQKT YIGNLLALSI SSDNLVNKYL IENFSAQLQN PEGKSFYGFQ
130 140 150 160 170 180
IMMENIYSEV YSMMVDAFFK DPKNIPLFKE IANLPEVKHK AAFIERWISN DDSLYAERLV
190 200 210 220 230 240
AFAAKEGIFQ AGNYASMFWL TDKKIMPGLA MANRNICRDR GAYTDFSCLL FAHLRTKPNP
250 260 270 280 290 300
KIIEKIITEA VEIEKEYYSN SLPVEKFGMD LKSIHTYIEF VADGLLQGFG NEKYYNAVNP
310 320 330 340
FEFMEDVATA GKTTFFEKKV SDYQKASDMS KSATPSKEIN FDDDF
References and
documentation are available.
Number of amino acids: 345
Molecular weight: 40054.61
Theoretical pI: 5.11
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1819
Hydrogen H 2763
Nitrogen N 451
Oxygen O 537
Sulfur S 16
Formula: C
1819H
2763N
451O
537S
16
Total number of atoms: 5586
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 45965
Abs 0.1% (=1 g/l) 1.148, assuming all pairs of Cys residues form cystines
Ext. coefficient 45840
Abs 0.1% (=1 g/l) 1.144, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.74
This classifies the protein as unstable.
Aliphatic index: 73.88
Grand average of hydropathicity (GRAVY): -0.418