ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEALIVFIVL SVSGAFAYKC SYERLWFKVG SLFDIISTSS KKNVIPLASK MEVGSNEDVS 

        70         80         90        100        110        120 
SMGNFINKFY TEYSLPSHKV LQSLRVLFSL AMMTYTVTIE IILWQIKVAG MDKEVTFITT 

       130        140        150        160        170        180 
WVWPLTAIML SFILILFQPF FIIISLLNKF YNDKFDIDRL IIVTCIILST LIALLSYINI 

       190        200        210        220        230        240 
GPFQYTKNIL TRLSIGGVTV MASLSGLATV SSLYYNFLVI WHKFRNTPMS DPSFRNINNS 

       250        260        270        280        290        300 
NNNSKSLLWT TDAYIEEKIQ DYEHNIEQNV QILRSLEEEV GENSTFKAEL MEKIAWYQLE 

       310        320        330        340        350        360 
LGKLEALLQQ SPQVRTFKKA FEVGFIIYCL HKLIITFLKR IPYIIYHSLK YPDDYDYENF 

       370        380        390        400        410        420 
SENAASDPLA ITIANILDFS FFRFNYQHDL DSLTKQISLF LSISLFLCCL SAVNTTISYV 

       430        440        450        460        470        480 
VTLLPIKFQI LALFAMQNDD TANVLPEYTN NSSYKGKKRN YSQEQKGISL IKNLVVSELT 

       490        500        510        520        530        540 
GVYVLATTLM VRSHLPFEVS QRLKELLGGK FTVPNIVIDS WFDEVYAFAC VFTFICIRIA 

       550 
ERKLSTKKVS VE 

References and documentation are available.

Number of amino acids: 552

Molecular weight: 63330.88

Theoretical pI: 7.04

Amino acid composition: 
Ala (A)  27	  4.9%
Arg (R)  15	  2.7%
Asn (N)  31	  5.6%
Asp (D)  20	  3.6%
Cys (C)   7	  1.3%
Gln (Q)  18	  3.3%
Glu (E)  30	  5.4%
Gly (G)  17	  3.1%
His (H)   7	  1.3%
Ile (I)  56	 10.1%
Leu (L)  66	 12.0%
Lys (K)  35	  6.3%
Met (M)  12	  2.2%
Phe (F)  38	  6.9%
Pro (P)  15	  2.7%
Ser (S)  52	  9.4%
Thr (T)  34	  6.2%
Trp (W)   8	  1.4%
Tyr (Y)  26	  4.7%
Val (V)  38	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2935
Hydrogen    H	      4563
Nitrogen    N	       703
Oxygen      O	       814
Sulfur      S	        19

Formula: C2935H4563N703O814S19
Total number of atoms: 9034

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    83115
Abs 0.1% (=1 g/l)   1.312, assuming all pairs of Cys residues form cystines


Ext. coefficient    82740
Abs 0.1% (=1 g/l)   1.306, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.91
This classifies the protein as unstable.



Aliphatic index: 111.05

Grand average of hydropathicity (GRAVY): 0.267