ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEAMLVDCVN NSLRHFVYKN AIFMCERLCA EFPSEVNLQL LATSYLQNNQ AYSAYHLLKG 

        70         80         90        100        110        120 
TQMAQSRYLF ALSCFQMDLL NEAESALCPV NEPGAEIPNG AAGHYLLGLI YKYTDRRKNA 

       130        140        150        160        170        180 
AQQFKQSLTI DPLLWAAYEE LCILGAAEEA TAVFGETAAL SIQKQYMQQL STSLGLNTYN 

       190        200        210        220        230        240 
EERNSTSTKN TSSEDYSPRQ SKHTQSHGLK DISGNFHSHG VNGGVSNMSF YNTPSPVAAQ 

       250        260        270        280        290        300 
LSGIAPPPLF RNFQPAVANP NSLITDSSPK STVNSTLQAP RRKFVDEGKL RKISGRLFSD 

       310        320        330        340        350        360 
SGPRRSSRLS ADSGANINSS VATVSGNVNN ASKYLGGSKL SSLALRSVTL RKGHSWANEN 

       370        380        390        400        410        420 
MDEGVRGEPF DDSRPNTAST TGSMASNDQE DETMSIGGIA MSSQTITIGV SEILNLLRTL 

       430        440        450        460        470        480 
GEGCRLSYMY RCQEALDTYM KLPHKHYNTG WVLSQVGKAY FELIDYLEAE KAFRLARLAS 

       490        500        510        520        530        540 
PYCLEGMDIY STVLYHLKED MKLSYLAQEL ISTDRLAPQS WCAMGNCYSL QKDHETALKN 

       550        560        570        580        590        600 
FLRAVQLNPR FAYAHTLCGH EYTTLEDFEN GMKSYQNALR VDTRHYNAWY GLGMIYLRQE 

       610        620        630        640        650        660 
KLEFSEHHFR MAFLINPSSS VIMSYLGTSL HALKRSEEAL EIMEQAIVAD RKNPLPMYQK 

       670        680        690        700        710        720 
ANILVCLERL DEALEVLEEL KEYAPSESSV YALMGRIYKR RNMHDKAMLH FGLALDMKPP 

       730        740 
ATDVAAIKAA MEKLHVPDEI DESP 

References and documentation are available.

Number of amino acids: 744

Molecular weight: 83068.07

Theoretical pI: 6.12

Amino acid composition: 
Ala (A)  69	  9.3%
Arg (R)  37	  5.0%
Asn (N)  43	  5.8%
Asp (D)  30	  4.0%
Cys (C)  13	  1.7%
Gln (Q)  30	  4.0%
Glu (E)  52	  7.0%
Gly (G)  41	  5.5%
His (H)  21	  2.8%
Ile (I)  28	  3.8%
Leu (L)  85	 11.4%
Lys (K)  35	  4.7%
Met (M)  27	  3.6%
Phe (F)  23	  3.1%
Pro (P)  30	  4.0%
Ser (S)  73	  9.8%
Thr (T)  36	  4.8%
Trp (W)   5	  0.7%
Tyr (Y)  36	  4.8%
Val (V)  30	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 82
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3650
Hydrogen    H	      5722
Nitrogen    N	      1010
Oxygen      O	      1127
Sulfur      S	        40

Formula: C3650H5722N1010O1127S40
Total number of atoms: 11549

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    81890
Abs 0.1% (=1 g/l)   0.986, assuming all pairs of Cys residues form cystines


Ext. coefficient    81140
Abs 0.1% (=1 g/l)   0.977, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.82
This classifies the protein as unstable.



Aliphatic index: 80.20

Grand average of hydropathicity (GRAVY): -0.356