ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MECVSVEGLD SSFLEGQTFG DILCLPWTII KGIRERKNRN KMKIILKNVS LLAKSGEMVL 

        70         80         90        100        110        120 
VLGRPGAGCT SFLKSAAGET SQFAGGVTTG HISYDGIPQK EMMQHYKPDV IYNGEQDVHF 

       130        140        150        160        170        180 
PHLTVKQTLD FAISCKMPAK RVNNVTKEEY ITANREFYAK IFGLTHTFDT KVGNDFISGV 

       190        200        210        220        230        240 
SGGERKRVSI AEALAAKGSI YCWDNATRGL DSSTALEFAR AIRTMTNLLG TTALVTVYQA 

       250        260        270        280        290        300 
SENIYETFDK VTVLYAGRQI FCGKTTEAKD YFENMGYLCP PRQSTAEYLT AITDPNGLHE 

       310        320        330        340        350        360 
IKPGFEYQVP HTADEFEKYW LDSPEYARLK GEIQKYKHEV NTEWTKKTYN ESMAQEKSKG 

       370        380        390        400        410        420 
TRKKSYYTVS YWEQIRLCTI RGFLRIYGDK SYTVINTCAA IAQAFITGSL FYQAPSSTLG 

       430        440        450        460        470        480 
AFSRSGVLFF SLLYYSLMGL ANISFEHRPI LQKHKVYSLY HPSAEALAST ISSFPFRMIG 

       490        500        510        520        530        540 
LTFFIIILYF LAGLHRSAGA FFTMYLLLTM CSEAITSLFQ MVSSLCDTLS QANSIAGVVM 

       550        560        570        580        590        600 
LSIAMYSTYM IQLPSMHPWF KWISYILPIR YAFESMLNAE FHGRHMDCGG TLVPSGPGFE 

       610        620        630        640        650        660 
NILPENQVCA FVGSRPGQSW VLGDDYLRAQ YQYEYKNTWR NFGIMWCFLI GYIVLRAVFT 

       670        680        690        700        710        720 
EYKSPVKSGG DALVVKKGTK NAIQRSWSSK NDEENLNASI ATQDMKEIAS SNDDSTSADF 

       730        740        750        760        770        780 
EGLESTGVFI WKNVSFTIPH SSGQRKLLDS VSGYCVPGTL TALIGESGAG KTTLLNTLAQ 

       790        800        810        820        830        840 
RNVGTITGDM LVDGLPMDAS FKRRTGYVQQ QDLHVAELTV KESLQFSARM RRPQSIPDAE 

       850        860        870        880        890        900 
KMEYVEKIIS ILEMQEFSEA LVGEIGYGLN VEQRKKLSIG VELVGKPDLL LFLDEPTSGL 

       910        920        930        940        950        960 
DSQSAWAVVK MLKRLALAGQ SILCTIHQPS ATLFEQFDRL LLLGKGGQTI YFGEIGKNSS 

       970        980        990       1000       1010       1020 
SVIKYFEKNG ARKCQQNENP AEYILEAIGA GATASVQQNW PDIWQKSHEY ANINEKINDM 

      1030       1040       1050       1060       1070       1080 
IKDLSSTTLH KTATRASKYA TSYSYQFHHV LKRSSLTFWR NLNYIMAKMM LLMISGLFIG 

      1090       1100       1110       1120       1130       1140 
FTFFHVGVNA IGLQNSLFAC FMAIVISAPA TNQIQERATV AKELYEVRES KSNMFHWSLL 

      1150       1160       1170       1180       1190       1200 
LITHYLNELP YHLLFSTIFF VSSYFPLGVF TEASRSSVFY LNYAILFQLY YIGLALMILY 

      1210       1220       1230       1240       1250       1260 
MSPNLQSANV IVGFILSFLL SFCGAVQPAS LMPGFWTFMW KLSPYTYFLQ NLVGLLMHDK 

      1270       1280       1290       1300       1310       1320 
PVRCSKKELS LFNPPVGQTC GEFTKPFFEF GTGYIANPDA TADCAYCQYK VGDEYLARIN 

      1330 
ASFSYLWRNF GFI 

References and documentation are available.

Number of amino acids: 1333

Molecular weight: 149750.13

Theoretical pI: 8.19

Amino acid composition: 
Ala (A)  97	  7.3%
Arg (R)  49	  3.7%
Asn (N)  55	  4.1%
Asp (D)  43	  3.2%
Cys (C)  23	  1.7%
Gln (Q)  54	  4.1%
Glu (E)  75	  5.6%
Gly (G)  95	  7.1%
His (H)  27	  2.0%
Ile (I)  86	  6.5%
Leu (L) 131	  9.8%
Lys (K)  75	  5.6%
Met (M)  40	  3.0%
Phe (F)  80	  6.0%
Pro (P)  46	  3.5%
Ser (S) 115	  8.6%
Thr (T)  85	  6.4%
Trp (W)  20	  1.5%
Tyr (Y)  67	  5.0%
Val (V)  70	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 118
Total number of positively charged residues (Arg + Lys): 124

Atomic composition:

Carbon      C	      6803
Hydrogen    H	     10458
Nitrogen    N	      1738
Oxygen      O	      1946
Sulfur      S	        63

Formula: C6803H10458N1738O1946S63
Total number of atoms: 21008

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   211205
Abs 0.1% (=1 g/l)   1.410, assuming all pairs of Cys residues form cystines


Ext. coefficient   209830
Abs 0.1% (=1 g/l)   1.401, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.06
This classifies the protein as stable.



Aliphatic index: 85.99

Grand average of hydropathicity (GRAVY): -0.038