ProtParam
User-provided sequence:
10 20 30 40 50 60
MEDFDDDSFN DVLGAMEMPT AAKQPKVDAP AATAVNPAPS NSTEPSGSGR PAPGKPASNP
70 80 90 100 110 120
HCVLVHSKQR GNPILKSILN VPLEFRDDIV PDYVVGRTSC VLYLSLKYHN LNPDYICQRL
130 140 150 160 170 180
KALGKMYELR VLLVQVDTPE PNNALKSLTR ISLLADLTMM LAWNAEEAGK IIETYKQFEK
190 200 210 220 230 240
RPPDLIMERV ESNPHQKLLA ALTNIKPVNK TDAAALLHTF GNLGNIINAS EERLSQVMGL
250
GPRKAKKLYK TLQEPFLSK
References and
documentation are available.
Number of amino acids: 259
Molecular weight: 28583.00
Theoretical pI: 8.32
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 1269
Hydrogen H 2050
Nitrogen N 348
Oxygen O 378
Sulfur S 11
Formula: C
1269H
2050N
348O
378S
11
Total number of atoms: 4056
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 16055
Abs 0.1% (=1 g/l) 0.562, assuming all pairs of Cys residues form cystines
Ext. coefficient 15930
Abs 0.1% (=1 g/l) 0.557, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.76
This classifies the protein as unstable.
Aliphatic index: 91.54
Grand average of hydropathicity (GRAVY): -0.326