ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDGKQAINE GSNDASPDLD VNGTILMNNE DFSQWSVDDV ITWCISTLEV EETDPLCQRL 

        70         80         90        100        110        120 
RENDIVGDLL PELCLQDCQD LCDGDLNKAI KFKILINKMR DSKLEWKDDK TQEDMITVLK 

       130        140        150        160        170        180 
NLYTTTSAKL QEFQSQYTRL RMDVLDVMKT SSSSSPINTH GVSTTVPSSN NTIIPSSDGV 

       190        200        210        220        230        240 
SLSQTDYFDT VHNRQSPSRR ESPVTVFRQP SLSHSKSLHK DSKNKVPQIS TNQSHPSAVS 

       250        260        270        280        290        300 
TANTPGPSPN EALKQLRASK EDSCERILKN AMKRHNLADQ DWRQYVLVIC YGDQERLLEL 

       310        320        330        340 
NEKPVIIFKN LKQQGLHPAI MLRRRGDFEE VAMMNGSDNV TPGGRL 

References and documentation are available.

Number of amino acids: 346

Molecular weight: 38970.74

Theoretical pI: 5.29

Amino acid composition: 
Ala (A)  12	  3.5%
Arg (R)  18	  5.2%
Asn (N)  23	  6.6%
Asp (D)  30	  8.7%
Cys (C)   7	  2.0%
Gln (Q)  20	  5.8%
Glu (E)  20	  5.8%
Gly (G)  14	  4.0%
His (H)   7	  2.0%
Ile (I)  18	  5.2%
Leu (L)  33	  9.5%
Lys (K)  22	  6.4%
Met (M)  10	  2.9%
Phe (F)   7	  2.0%
Pro (P)  17	  4.9%
Ser (S)  36	 10.4%
Thr (T)  22	  6.4%
Trp (W)   4	  1.2%
Tyr (Y)   5	  1.4%
Val (V)  21	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1673
Hydrogen    H	      2697
Nitrogen    N	       483
Oxygen      O	       553
Sulfur      S	        17

Formula: C1673H2697N483O553S17
Total number of atoms: 5423

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29825
Abs 0.1% (=1 g/l)   0.765, assuming all pairs of Cys residues form cystines


Ext. coefficient    29450
Abs 0.1% (=1 g/l)   0.756, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 55.18
This classifies the protein as unstable.



Aliphatic index: 78.55

Grand average of hydropathicity (GRAVY): -0.663