ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDHGIVETL NFLSSTKIKE RNNALDELTT ILKEDPERIP TKALSTTAEA LVELLASEHT 

        70         80         90        100        110        120 
KYCDLLRNLT VSTTNKLSLS ENRLSTISYV LRLFVEKSCE RFKVKTLKLL LAVVPELMVK 

       130        140        150        160        170        180 
DGSKSLLDAV SVHLSFALDA LIKSDPFKLK FMIHQWISLV DKICEYFQSQ MKLSMVDKTL 

       190        200        210        220        230        240 
TNFISILLNL LALDTVGIFQ VTRTITWTVI DFLRLSKKEN GNTRLIMSLI NQLILKCHCF 

       250        260        270        280        290        300 
SVIDTLMLIK EAWSYNLTIG CTSNELVQDQ LSLFDVMSSE LMNHKLPYMI GQENYVEELR 

       310        320        330        340        350        360 
SESLVSLYRE YILLRLSNYK PQLFTVNHVE FSYIRGSRDK NSWFALPDFR LRDRGGRSVW 

       370        380        390        400        410        420 
LKILGITKSL LTYFALNRKN ENYSLLFKRR KCDSDIPSIL RISDDMDTFL IHLLEENSSH 

       430        440        450        460        470        480 
EFEVLGLQLC SFYGTLQDFT KSFAEQLKEL LFSKFEKIQC FNWVCFSFIP LLSQKECELS 

       490        500        510        520        530        540 
NGDMARLFKV CLPLVKSNES CQLSCLLLAN SIKFSKQLLS DEKTINQIYD LYELSDILGP 

       550        560        570        580        590        600 
ILVTNESFML WGYLQYVGKD FQSMNGISSA DRIFEWLKSK WNQLRGTDAK QDQFCNFISW 

       610        620        630        640        650        660 
LGNKYDPENP FNDKKGEGAN PVSLCWDESH KIWQHFQEQR EFLLGVKPEE KSECFNTPFF 

       670        680        690        700        710        720 
NLPKVSLDLT RYNEILYRLL ENIESDAFSS PLQKFTWVAK LIQIVDNLCG DSTFSEFIAA 

       730        740        750        760        770        780 
YKRTTLITIP QLSFDSQNSY QSFFEEVLSI RTINVDHLVL DKINMKEIVN DFIRMQKNKS 

       790        800        810        820        830        840 
QTGTSAINYF EASSEDTTQN NSPYTIGGRF QKPLHSTIDK AVRAYLWSSR NKSISERLVA 

       850        860        870        880        890        900 
ILEFSDCVST DVFISYLGTV CQWLKQAIGE KSSYNKILEE FTEVLGEKLL CNHYSSSNQA 

       910        920        930        940        950        960 
MLLLTSYIEA IRPQWLSYPE QPLNSDCNDI LDWIISRFED NSFTGVAPTV NLSMLLLSLL 

       970        980        990       1000       1010       1020 
QNHDLSHGSI RGGKQRVFAT FIKCLQKLDS SNIINIMNSI SSYMAQVSYK NQSIIFYEIK 

      1030       1040       1050       1060       1070       1080 
SLFGPPQQSI EKSAFYSLAM SMLSLVSYPS LVFSLEDMMT YSGFNHTRAF IQQALNKITV 

      1090       1100       1110       1120       1130       1140 
AFRYQNLTEL FEYCKFDLIM YWFNRTKVPT SKLEKEWDIS LFGFADIHEF LGRYFVEISA 

      1150       1160       1170       1180       1190       1200 
IYFSQGFNQK WILDMLHAIT GNGDAYLVDN SYYLCIPLAF ISGGVNELIF DILPQISGKT 

      1210       1220       1230       1240       1250       1260 
TVKYHKKYRL LMLKWIIRFT DLGSLTELRS TVEKLFPTSY LSPYLFENSS VSMRYQYPLH 

      1270       1280       1290       1300       1310       1320 
IPLALGATLV QTQFAHEKNN THEFKLLFLS VITDLEKTST YIGKLRCARE LKYLFVLYEN 

      1330       1340       1350       1360       1370       1380 
VLVKSSTLNF IIIRLSKFLI DTQIHDEVIT IFSSLLNLAD KNTFEIEPSL PNLFCKIFIY 

      1390       1400       1410       1420       1430       1440 
LRENKQLSPS FQQAIKLLEH RDLIKIKTWK YCLDAIFGNI VQDDIYENTE LLDASDCGVD 

      1450       1460       1470       1480       1490       1500 
DVVLVSLLFS YARRPVASKI GCSLSKAAAI NILKHHVPKE YLSKNFKLWF AALSRRILQQ 

      1510       1520       1530       1540       1550       1560 
EVQRERSTNF NNEVHLKNFE MVFRHPEQPH MIYQRISTFN KEAELYDSTE VFFISECILT 

      1570       1580       1590       1600       1610       1620 
YLVGYSIGNS ESEFCFRDNI MNENKDKVAP LDKDVLNAIY PLANNFGMES FICDTYLSVN 

      1630       1640       1650       1660       1670       1680 
EPYNCWLSKF ARSLIHQISF NIPPIVCLYP LCKGSTAFCE LVLTDLFFLS TTYDPKSCLN 

      1690       1700       1710       1720       1730       1740 
WSNRIFTQIA MLLHVKDSEI KLKMLFNVIK MIRMGSRCKE RNCLRIYSSL DLQEICQISL 

      1750       1760       1770       1780       1790       1800 
KIKEFKFGYL LFEEMNMPNI REMNINTLQK IYECINDGDF LAGLPVPHSI EGVLNSINRI 

      1810       1820       1830       1840       1850       1860 
DSDTWKRFLF NNADFDANYT TSLEEEKESL IKATEDSGFY GLTSLLESRL SGSSDVYKWN 

      1870       1880       1890       1900       1910       1920 
LELGDWKLLT PKVVDSKAKG LYYAIKNLPQ DVGFAEKSLE KSLLTIFDSR QHFISQTEWM 

      1930       1940       1950       1960       1970       1980 
DTLNAIIEFI KIAAIPQDVT SFPQTLMSIM KADKERLNTI DFYDHKTTLK SRHTLMNVLS 

      1990       2000       2010       2020       2030       2040 
RNSLDENVKC SKYLRLGSII QLANYVQLAI ANGAPQDALR NATLMSKTVK NIAKLYDDPS 

      2050       2060       2070       2080       2090       2100 
VVSQIEKLAS FTSANALWES REYKAPVMIM RDLLAQNEKN ISESILYDDF KLLINVPMDQ 

      2110       2120       2130       2140       2150       2160 
IKARLVKWSS ESRLEPAAAI YEKIIVNWDI NVEDHESCSD VFYTLGSFLD EQAQKLRSNG 

      2170       2180       2190       2200       2210       2220 
EIEDREHRSY TGKSTLKALE LIYKNTKLPE NERKDAKRHY NRVLLQYNRD SEVLKALLLQ 

      2230       2240       2250       2260       2270       2280 
KEKFLWHALH FYLNTLVFSN RYDNDIIDKF CGLWFENDDN SKINQLLYKE IGTIPSWKFL 

      2290       2300       2310       2320       2330       2340 
PWVNQIASKI SMEENEFQKP LQLTMKRLLY KLPYDSLYSV MSILLYEKQS NKDTNISQKI 

      2350       2360       2370       2380       2390       2400 
QAVKKILLEL QGYDRGAFAK KYLLPVQEFC EMSVELANLK FVQNTKTLRL ANLKIGQYWL 

      2410       2420       2430       2440       2450       2460 
KQLNMEKLPL PTSNFTVKSS ADGRKARPYI VSVNETVGIT TTGLSLPKIV TFNISDGTTQ 

      2470       2480       2490       2500       2510       2520 
KALMKGSNDD LRQDAIMEQV FQQVNKVLQN DKVLRNLDLG IRTYKVVPLG PKAGIIEFVA 

      2530       2540       2550       2560       2570       2580 
NSTSLHQILS KLHTNDKITF DQARKGMKAV QTKSNEERLK AYLKITNEIK PQLRNFFFDS 

      2590       2600       2610       2620       2630       2640 
FPDPLDWFEA KKTYTKGVAA SSIVGYILGL GDRHLNNILL DCSTGEPIHI DLGIAFDQGK 

      2650       2660       2670       2680       2690       2700 
LLPIPELVPF RLTRDIVDGF GVTGVDGLFR RSCERVYAVL RKDYVKVMCV LNILKWDPLY 

      2710       2720       2730       2740       2750       2760 
SWVMSPVKKY EHLFEEEHEI TNFDNVSKFI SNNDRNENQE SYRALKGVEE KLMGNGLSVE 

      2770       2780 
SSVQDLIQQA TDPSNLSVIY MGWSPFY 

References and documentation are available.

Number of amino acids: 2787

Molecular weight: 321524.49

Theoretical pI: 6.52

Amino acid composition: 
Ala (A) 120	  4.3%
Arg (R) 109	  3.9%
Asn (N) 170	  6.1%
Asp (D) 149	  5.3%
Cys (C)  49	  1.8%
Gln (Q) 112	  4.0%
Glu (E) 179	  6.4%
Gly (G)  98	  3.5%
His (H)  47	  1.7%
Ile (I) 206	  7.4%
Leu (L) 357	 12.8%
Lys (K) 207	  7.4%
Met (M)  59	  2.1%
Phe (F) 157	  5.6%
Pro (P)  84	  3.0%
Ser (S) 251	  9.0%
Thr (T) 140	  5.0%
Trp (W)  41	  1.5%
Tyr (Y) 109	  3.9%
Val (V) 143	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 328
Total number of positively charged residues (Arg + Lys): 316

Atomic composition:

Carbon      C	     14578
Hydrogen    H	     22814
Nitrogen    N	      3738
Oxygen      O	      4226
Sulfur      S	       108

Formula: C14578H22814N3738O4226S108
Total number of atoms: 45464

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   390910
Abs 0.1% (=1 g/l)   1.216, assuming all pairs of Cys residues form cystines


Ext. coefficient   387910
Abs 0.1% (=1 g/l)   1.206, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.93
This classifies the protein as stable.



Aliphatic index: 97.97

Grand average of hydropathicity (GRAVY): -0.165