ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDIHNLDIE KSDRSIWLMK CPVVVDKAWH KIAASSSSSF ASSDSPPDMA KIVREVDPLR 

        70         80         90        100        110        120 
DDSPPEFKMY MVGAEYGNMP KCYALNMFTD FVPMGGFSDV NQGCAAAEGK VDHKFDMKPY 

       130        140        150        160        170        180 
GETIEEYARL CRERTSKAMV KNRQIQVIDN DRGVHMRPMP GMLGLVSSNS KEKRKPPPVK 

       190        200        210        220        230        240 
QTEVKRTRRD RGELEAIMFK LFEGQPNWTL KQLVQETDQP AQFLKEILNE LCVYNKRGSN 

       250        260 
QGTYELKPEY KKSAEDDTGG Q 

References and documentation are available.

Number of amino acids: 261

Molecular weight: 29710.88

Theoretical pI: 6.47

Amino acid composition: 
Ala (A)  15	  5.7%
Arg (R)  15	  5.7%
Asn (N)  11	  4.2%
Asp (D)  19	  7.3%
Cys (C)   5	  1.9%
Gln (Q)  11	  4.2%
Glu (E)  21	  8.0%
Gly (G)  16	  6.1%
His (H)   4	  1.5%
Ile (I)  10	  3.8%
Leu (L)  15	  5.7%
Lys (K)  24	  9.2%
Met (M)  14	  5.4%
Phe (F)   9	  3.4%
Pro (P)  17	  6.5%
Ser (S)  18	  6.9%
Thr (T)   9	  3.4%
Trp (W)   3	  1.1%
Tyr (Y)   8	  3.1%
Val (V)  17	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1296
Hydrogen    H	      2051
Nitrogen    N	       363
Oxygen      O	       399
Sulfur      S	        19

Formula: C1296H2051N363O399S19
Total number of atoms: 4128

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    28670
Abs 0.1% (=1 g/l)   0.965, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.957, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.73
This classifies the protein as unstable.



Aliphatic index: 61.99

Grand average of hydropathicity (GRAVY): -0.742