ProtParam
User-provided sequence:
10 20 30 40 50 60
MEDKRKRRAA TLSTALILFV ACCVYTLYIF KFDNPRLSPP VSLLPTISTL KKIEHVTDLN
70 80 90 100 110 120
KEYVFVGDVH GNYDEFIELI DDKIGGLGEN ITMILLGDFI HKGPDSDKVV SYILNHKDQV
130 140 150 160 170 180
KCVLGNHEIL VMMAYLNPDF SKWVRRPKLM TPLTFSTETN FIPQDISKIS NAHGRLAREL
190 200 210 220 230 240
GFSKLSQLAE HCSMAIELDL DITGDILFGA HAGMVPGDFM KPNQIPGVSS LSNMKYVDKK
250 260 270 280 290 300
NWSKTSREKE NKNYVRWYTL WDKYGDHFSN AKVFYGHDAS MGLNLRRQTK GLDTACIKNN
310 320
LLSSMKVKYD IKKGQYDYEL IQVQCS
References and
documentation are available.
Number of amino acids: 326
Molecular weight: 37150.90
Theoretical pI: 8.55
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 1670
Hydrogen H 2624
Nitrogen N 442
Oxygen O 482
Sulfur S 17
Formula: C
1670H
2624N
442O
482S
17
Total number of atoms: 5235
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 43235
Abs 0.1% (=1 g/l) 1.164, assuming all pairs of Cys residues form cystines
Ext. coefficient 42860
Abs 0.1% (=1 g/l) 1.154, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.50
This classifies the protein as stable.
Aliphatic index: 89.08
Grand average of hydropathicity (GRAVY): -0.286