ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDPFYEVKA DASNQMEQVR KLYNSFMAAR NSGVLSPNTE LTYAIDELSE TLKDLKAAVE 

        70         80         90        100        110        120 
IAMKNSEHFG LDEEELKSRR RFVSELDIEL NNIQLKMGAA PSTPVSTEPY TVDNGASAGL 

       130        140        150        160        170        180 
SEEDHAVNRQ YQEQLYQQQD VMLDGVYDTI GNIRGQAALM GEELGQQADL LDTLDNSIET 

       190        200        210        220 
TNSKLRRGMK RLKDFTIASA DSKSGCCITV LIIILIALLV LVIVL 

References and documentation are available.

Number of amino acids: 225

Molecular weight: 24970.25

Theoretical pI: 4.53

Amino acid composition: 
Ala (A)  19	  8.4%
Arg (R)  10	  4.4%
Asn (N)  12	  5.3%
Asp (D)  16	  7.1%
Cys (C)   2	  0.9%
Gln (Q)  12	  5.3%
Glu (E)  20	  8.9%
Gly (G)  12	  5.3%
His (H)   2	  0.9%
Ile (I)  14	  6.2%
Leu (L)  27	 12.0%
Lys (K)  11	  4.9%
Met (M)   8	  3.6%
Phe (F)   5	  2.2%
Pro (P)   5	  2.2%
Ser (S)  17	  7.6%
Thr (T)  12	  5.3%
Trp (W)   0	  0.0%
Tyr (Y)   7	  3.1%
Val (V)  14	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1085
Hydrogen    H	      1758
Nitrogen    N	       294
Oxygen      O	       358
Sulfur      S	        10

Formula: C1085H1758N294O358S10
Total number of atoms: 3505

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10555
Abs 0.1% (=1 g/l)   0.423, assuming all pairs of Cys residues form cystines


Ext. coefficient    10430
Abs 0.1% (=1 g/l)   0.418, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.56
This classifies the protein as unstable.



Aliphatic index: 97.56

Grand average of hydropathicity (GRAVY): -0.246