ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDQSNAAPA VAAENGGNTA QEVSSTTQPI EPTKRSLRVQ TWKKIQEGKV GIGFNNIFNR 

        70         80         90        100        110        120 
IPSFVGADKA AALFANEEEF KKAQHIKVNI DRVLHEFKEL ALLADKSVYL PSTRESSALC 

       130        140        150        160        170        180 
LKVDVLADAT EEQKKEALRV QDIQKFRSEI GLDSGLKLDI VVIGSVVVSR EGYRIGRGNG 

       190        200        210        220        230        240 
FADLDIGLLI ELGAITPKTI IVTIVHDMQV VDSLPPNLFQ KYDTPVDIIA TPTEIIRVPK 

       250        260        270        280        290        300 
RLSFPNGVYW ELLSERRLKI LPVLQQLKER EEKAGKSISL KEEDTDVEQH QNNRRRRGPV 

       310        320        330        340        350        360 
RRRIQRGNPG RTTSQTDNEQ PQGETAQKRT PRRKGRFVNR RRRTTKSEGD QSGVEAGAKT 

       370        380        390        400        410        420 
DDRKLPEAGT DERRSKKNKN SDFCIKLTNL SRDIRVKDLK SELRKRECTP MSISWKGHFG 

       430        440        450        460        470        480 
KCFLHFGNRK GVPSTQDDMD KVLKSLNDLS LTITTGGETA PAAAAGEGGE KAESAETATP 

       490        500        510        520        530        540 
VQTKTILVNV ELIQFGEKKS ASSTGANAGA GEDGKADAQN GGEAVTVAGG DVGGARIEAV 


DTTTV 

References and documentation are available.

Number of amino acids: 545

Molecular weight: 59742.47

Theoretical pI: 9.12

Amino acid composition: 
Ala (A)  44	  8.1%
Arg (R)  41	  7.5%
Asn (N)  24	  4.4%
Asp (D)  33	  6.1%
Cys (C)   4	  0.7%
Gln (Q)  25	  4.6%
Glu (E)  44	  8.1%
Gly (G)  46	  8.4%
His (H)   6	  1.1%
Ile (I)  32	  5.9%
Leu (L)  40	  7.3%
Lys (K)  45	  8.3%
Met (M)   4	  0.7%
Phe (F)  16	  2.9%
Pro (P)  22	  4.0%
Ser (S)  34	  6.2%
Thr (T)  39	  7.2%
Trp (W)   3	  0.6%
Tyr (Y)   4	  0.7%
Val (V)  39	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 86

Atomic composition:

Carbon      C	      2589
Hydrogen    H	      4257
Nitrogen    N	       777
Oxygen      O	       826
Sulfur      S	         8

Formula: C2589H4257N777O826S8
Total number of atoms: 8457

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22710
Abs 0.1% (=1 g/l)   0.380, assuming all pairs of Cys residues form cystines


Ext. coefficient    22460
Abs 0.1% (=1 g/l)   0.376, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.50
This classifies the protein as unstable.



Aliphatic index: 80.35

Grand average of hydropathicity (GRAVY): -0.614