ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDSAQDAVT AAPSGTPKSK LETLPREDLI KFAKKQMMLL QKAKARCTEL DKEVEELKSK 

        70         80         90        100        110        120 
PVDGGTDDMI KVLTERLDAL LLEKAETEQQ CLCLKKENIK MKQEVEDSVT KLEETQKEFE 

       130        140        150        160        170        180 
QSHRNYVREM ESVKNELIAV HSEHSKEKAA LQRDLEGAVH RQAELLEQLK SQSDSEDNVK 

       190        200        210        220        230        240 
KLQEEIQNIT AAFEEQTSCL QKQLEATSDE KQQEIIHLQK VIEDNAQHYQ KDINTFQEEI 

       250        260        270        280        290        300 
VQLRATHKEE VNELMSQMET LAKEHEAAVN KLKENRVTLC ETSETIPENY QCESESLNED 

       310        320        330        340        350        360 
TSDASQENQK CSVALQEDPF AEHTVYDKVR QLEDSLKELE SQHSILKDEV TYMNNLKLKL 

       370        380        390        400        410        420 
EMDAQHIKDE FFHEREDLEF KINELLLAKE EQSYVVEKLK YEREDLNRQL CCTVEQHNKE 

       430        440        450        460        470        480 
IQRLQEHHQK EISELSETFM SGSEKEKLAL MFEIQGLKEQ CENLQHEKQE VVLNYESLRE 

       490        500        510        520        530        540 
MMEILQTELG ESAGKISQEF ETMKQQQASD VHELQQKLRT AFNEKDALLE TINRLQGENE 

       550        560        570        580        590        600 
KLLSQELVSE LESTMKNLKA DNSMYLASLG QKDTLLQELE AKISSLAEEK DDFISKIKTS 

       610        620        630        640        650        660 
REEIDDLHQK WEREQKLSVE LREAAEQAAQ HNSELRQRVS ELTGKLDEIL REKSQNDQNI 

       670        680        690        700        710        720 
MVQMKTMTED QEALSSKIKS LYEENNRLHS EKVQLSRDLE ALQSQQDFAY KEHVAEFEKK 

       730        740        750        760        770        780 
LQLMVEERDD LNKLLENEQL QKSFVKTQLY EFLKQMRPSI LEDNEEEDVV TVLKAVGESL 

       790        800        810        820        830        840 
VTVKEEKHNL VFEYDARVLE LERRIKCLQE ESVVQCEELR ALVRDSEQEK ILLRKELDEV 

       850        860        870        880        890        900 
TSTKEALQCD ILEMKNTNEK TSLENQTLST RVEELSRSLH SKNEVHNEKD LVIEHENLRL 

       910        920        930        940        950        960 
SLEQRESELQ DVRAELMLLK DSLEKSPSVK NDQLSLVKEL EEKIESLEKE SKDKDEKISK 

       970        980        990       1000       1010       1020 
IKLVAVRAKK ELDSNRKEAQ TLRDELESVQ SEKDRLSASM KEFIQGAESY KNLLLEYDKQ 

      1030       1040       1050       1060       1070       1080 
SEQLDVEKER ANNFEHHIED LTKQLRDSTC QYEKLTSDNE DLLARIETLQ ANARLLEAQI 

      1090       1100       1110       1120       1130       1140 
LEVQRAKGVV EKELEAEKLQ KEQKIKEHVS TTNELEELQL QFQKEKKQLQ KTMQELELVK 

      1150       1160       1170       1180       1190       1200 
KDAQQTTLMN MEIADYERLM KELNQKLTNK NSKIEDLEQE MKIQKQKQET LQEEMTSLQS 

      1210       1220       1230       1240       1250       1260 
SVQHYEEKNA QIKQLLVKTK KELADAKQAE TDHLLLQASL KGELEASQQQ VEVYKIQLAE 

      1270       1280       1290       1300       1310       1320 
MTSEKHKIHE HLKTSAEQHQ RTLSAYQQRV VALQEESRTA KAEQAAVTSE FENYKVRVHN 

      1330       1340       1350       1360       1370       1380 
VLKQQKNKSV SQAETEGAKQ EREHLEMLID QLKIKLQDSQ NSLQISVSEF QTLQSEHDTL 

      1390       1400       1410       1420       1430       1440 
LERHNRMLQE TVTKEAELRE KLCSAQSENT MLKSEHAQTM CQLTSQNEAL RNSFRDQVRH 

      1450       1460       1470       1480       1490       1500 
LQDEHRKTVE TLQHQLSKVE TQLFQLKSEP PTKSPASSHQ PSKSLRERRT TDLPLLDMHT 

      1510       1520       1530       1540       1550       1560 
VTREEGEGME TTDSESVSSA GTHIQSLEQL LSSPDSKLER LTEASLWHTE FTKEELAEKL 

      1570       1580       1590       1600       1610       1620 
SSTTKSADHL NGLLRETEAT NAILMEQIKL LKSEIRRLER NQEREKSVAN LEYLKNVLLR 

      1630       1640       1650       1660       1670 
FIFLKPGSER ERLLPVIDTM LQLSPEEKGK LATVAQGEEE SASRSSGWAS YLHSWSGLR 

References and documentation are available.

Number of amino acids: 1679

Molecular weight: 195083.12

Theoretical pI: 5.05

Amino acid composition: 
Ala (A)  95	  5.7%
Arg (R)  75	  4.5%
Asn (N)  66	  3.9%
Asp (D)  77	  4.6%
Cys (C)  16	  1.0%
Gln (Q) 149	  8.9%
Glu (E) 267	 15.9%
Gly (G)  25	  1.5%
His (H)  48	  2.9%
Ile (I)  56	  3.3%
Leu (L) 220	 13.1%
Lys (K) 165	  9.8%
Met (M)  40	  2.4%
Phe (F)  28	  1.7%
Pro (P)  17	  1.0%
Ser (S) 135	  8.0%
Thr (T)  90	  5.4%
Trp (W)   4	  0.2%
Tyr (Y)  23	  1.4%
Val (V)  83	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 344
Total number of positively charged residues (Arg + Lys): 240

Atomic composition:

Carbon      C	      8387
Hydrogen    H	     13756
Nitrogen    N	      2384
Oxygen      O	      2831
Sulfur      S	        56

Formula: C8387H13756N2384O2831S56
Total number of atoms: 27414

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57270
Abs 0.1% (=1 g/l)   0.294, assuming all pairs of Cys residues form cystines


Ext. coefficient    56270
Abs 0.1% (=1 g/l)   0.288, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.13
This classifies the protein as unstable.



Aliphatic index: 84.10

Grand average of hydropathicity (GRAVY): -0.912