ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEDSDSNTDK KFFFKKRRID SYNYSDEEDN NSSMNSDMTY TNDALKTSSG NAPTISKLTK 

        70         80         90        100        110        120 
TYGIGAKLLS SMGYVAGKGL GKDGSGITTP IETQSRPMHN AGLGMFSNTN SSNYHSENED 

       130        140        150        160        170        180 
YLSSEDEVVE GIEQVKFNKT STEVLGEALL NDSGDMTIVR TLRELRLAGV QLPESILKEL 

       190        200        210        220        230        240 
DPLNAVPKPK KDVVVEILQE LLGIEKSLEA IRQRTSPLEV QVKEYYGQER LLSELEVTLR 

       250        260        270        280        290        300 
DESKHVSLYD KIGAILKLSD DELIDRLTSC LLRKELLIEF DLDHLEKPND ILDELTQIIE 

       310        320        330        340        350        360 
LLAYRMDTTS KFLNRTQTTI FKVIYPKLKK FWEGFDMTKS KIDSAITLLL DFQQVLSFIG 

       370        380        390        400        410        420 
CKEHIMEEFV YPKLLQELDN WELHDEVDHV SPRIWVLDFM VLIDDKIKDT IVDKIEAKFF 

       430        440        450        460        470        480 
AYCKNWYHRE SFCITNSDII FIKELICERR YYKILCKEFL PKFLDELWER HNDPIYELED 

       490        500        510        520        530        540 
WKEKQEWKEK DSGFFYFMKK LRSYTHYFHP KQYELMMRGT FNNINKILYQ WHLYSTVEDL 

       550        560        570        580        590        600 
HKSKWWLNWL MNTVFEHSLP TEIELSEIRK SYNIFAMSHR YHLDKSTLDE DFDLRQGLRN 

       610        620        630        640        650        660 
LMETQVIDDI SQSEQEPTYT VQNIPLGKVS SSFKDVVEDY CLEKGYLISK IPNRYTQLPY 

       670        680        690        700 
GRDQDCIVPL FEIRNGKKKM EVALKHDILW VEDSSGTFKP IYLWALDL 

References and documentation are available.

Number of amino acids: 708

Molecular weight: 83048.65

Theoretical pI: 5.14

Amino acid composition: 
Ala (A)  17	  2.4%
Arg (R)  28	  4.0%
Asn (N)  31	  4.4%
Asp (D)  55	  7.8%
Cys (C)   8	  1.1%
Gln (Q)  22	  3.1%
Glu (E)  65	  9.2%
Gly (G)  28	  4.0%
His (H)  17	  2.4%
Ile (I)  50	  7.1%
Leu (L)  83	 11.7%
Lys (K)  62	  8.8%
Met (M)  18	  2.5%
Phe (F)  31	  4.4%
Pro (P)  22	  3.1%
Ser (S)  54	  7.6%
Thr (T)  39	  5.5%
Trp (W)  13	  1.8%
Tyr (Y)  32	  4.5%
Val (V)  33	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 120
Total number of positively charged residues (Arg + Lys): 90

Atomic composition:

Carbon      C	      3743
Hydrogen    H	      5818
Nitrogen    N	       954
Oxygen      O	      1127
Sulfur      S	        26

Formula: C3743H5818N954O1127S26
Total number of atoms: 11668

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   119680
Abs 0.1% (=1 g/l)   1.441, assuming all pairs of Cys residues form cystines


Ext. coefficient   119180
Abs 0.1% (=1 g/l)   1.435, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.84
This classifies the protein as unstable.



Aliphatic index: 89.18

Grand average of hydropathicity (GRAVY): -0.491