ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEEDCEFRHW DELIPDALGL IFSHLPLQEV LTVVPRVCKA WNRAVTGPYC WQEIDIELWS 

        70         80         90        100        110        120 
NRFHQSDHLD RMLEMLIPRS AGSLRKLSVT GLRNDSIFSF IAQHAGSLKT LKVPRSGLTN 

       130        140        150        160        170        180 
SGVVNVAEKL SSLTFLDLSY CCKIGPEAIQ AIGKHCKSLR EFCRNMHPLD VASVVSHDDE 

       190        200        210        220        230        240 
AYAIANTMPK LKRLEIAYHR VSTEGVLKIL SCCVFLEFLE LRGCWDVQLD NKFFKEKFPD 

       250        260        270        280        290        300 
MKVLGPRVIG FYDMINDWED CCSDYFSDGS DYLAWEFFED GVMGEFYEDE FEHGWDDNFY 

       310 
AENAVLDMEP HIWPPSP 

References and documentation are available.

Number of amino acids: 317

Molecular weight: 36482.60

Theoretical pI: 4.86

Amino acid composition: 
Ala (A)  17	  5.4%
Arg (R)  15	  4.7%
Asn (N)  11	  3.5%
Asp (D)  25	  7.9%
Cys (C)  12	  3.8%
Gln (Q)   6	  1.9%
Glu (E)  26	  8.2%
Gly (G)  17	  5.4%
His (H)  11	  3.5%
Ile (I)  16	  5.0%
Leu (L)  33	 10.4%
Lys (K)  15	  4.7%
Met (M)   9	  2.8%
Phe (F)  19	  6.0%
Pro (P)  15	  4.7%
Ser (S)  23	  7.3%
Thr (T)   8	  2.5%
Trp (W)   9	  2.8%
Tyr (Y)   9	  2.8%
Val (V)  21	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1642
Hydrogen    H	      2483
Nitrogen    N	       425
Oxygen      O	       477
Sulfur      S	        21

Formula: C1642H2483N425O477S21
Total number of atoms: 5048

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63660
Abs 0.1% (=1 g/l)   1.745, assuming all pairs of Cys residues form cystines


Ext. coefficient    62910
Abs 0.1% (=1 g/l)   1.724, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.82
This classifies the protein as unstable.



Aliphatic index: 84.86

Grand average of hydropathicity (GRAVY): -0.181