ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEEEIAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH HGIMVGMGQK DSYVGDEAQS 

        70         80         90        100        110        120 
KRGILTLKYP IEHGIVNNWD DMEKIWHHTF YNELRVAPEE HPCLLTEAPL NPKSNREKMT 

       130        140        150        160        170        180 
QIIFETFNAP AFYVAIQAVL SLYASGRTTG IVLDSGDGVT HTVPIYEGYA LPHAIMRLDL 

       190        200        210        220        230        240 
AGRDLTDYLM KILMERGYTF STTAEREIVR DIKEKLCYVA LDFEQELQTA AQSSSLEKSY 

       250        260        270        280        290        300 
ELPDGQVITI GNERFRAPEA LFQPSALGLE NAGIHEATYN SIMKCDVDIR KDLYGNVVMS 

       310        320        330        340        350        360 
GGTTMYPGIA DRMQKEIQAL APSSMKVKIV APPERKYSVW IGGSILASLS TFQQMWISKQ 

       370 
EYDESGPGIV YRKCF 

References and documentation are available.

Number of amino acids: 375

Molecular weight: 41764.70

Theoretical pI: 5.31

Amino acid composition: 
Ala (A)  31	  8.3%
Arg (R)  18	  4.8%
Asn (N)  11	  2.9%
Asp (D)  20	  5.3%
Cys (C)   5	  1.3%
Gln (Q)  14	  3.7%
Glu (E)  29	  7.7%
Gly (G)  30	  8.0%
His (H)   9	  2.4%
Ile (I)  29	  7.7%
Leu (L)  28	  7.5%
Lys (K)  19	  5.1%
Met (M)  15	  4.0%
Phe (F)  12	  3.2%
Pro (P)  19	  5.1%
Ser (S)  24	  6.4%
Thr (T)  19	  5.1%
Trp (W)   4	  1.1%
Tyr (Y)  17	  4.5%
Val (V)  22	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1858
Hydrogen    H	      2907
Nitrogen    N	       495
Oxygen      O	       559
Sulfur      S	        20

Formula: C1858H2907N495O559S20
Total number of atoms: 5839

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47580
Abs 0.1% (=1 g/l)   1.139, assuming all pairs of Cys residues form cystines


Ext. coefficient    47330
Abs 0.1% (=1 g/l)   1.133, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.05
This classifies the protein as stable.



Aliphatic index: 84.56

Grand average of hydropathicity (GRAVY): -0.223