ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEEENEVVKT FAELGVREEL VKACERLGWK NPSKIQAEAL PFALEGKDVI GLAQTGSGKT 

        70         80         90        100        110        120 
GAFAIPILQA LLEYVYDSEP KKGRRPDPAF FACVLSPTRE LAIQIAEQFE ALGADISLRC 

       130        140        150        160        170        180 
AVLVGGIDRM QQTIALGKRP HVIVATPGRL WDHMSDTKGF SLKSLKYLVL DEADRLLNED 

       190        200        210        220        230        240 
FEKSLNQILE EIPLERKTFL FSATMTKKVR KLQRACLRNP VKIEAASKYS TVDTLKQQYR 

       250        260        270        280        290        300 
FVAAKYKDCY LVYILSEMPE STSMIFTRTC DGTRFLALVL RSLGFRAIPI SGQMTQSKRL 

       310        320        330        340        350        360 
GALNKFKAGE CNILVCTDVA SRGLDIPSVD VVINYDIPTN SKDYIHRVGR TARAGRSGVG 

       370        380        390        400        410        420 
ISLVNQYELE WYIQIEKLIG KKLPEYPAEE DEVLSLLERV AEAKKLSAMN MKESGGRKRR 

       430        440        450 
GEDDEESERF LGGNKDRGNK ERGGNKDKKS SKKFKR 

References and documentation are available.

Number of amino acids: 456

Molecular weight: 51147.02

Theoretical pI: 9.07

Amino acid composition: 
Ala (A)  36	  7.9%
Arg (R)  33	  7.2%
Asn (N)  14	  3.1%
Asp (D)  23	  5.0%
Cys (C)   8	  1.8%
Gln (Q)  15	  3.3%
Glu (E)  40	  8.8%
Gly (G)  33	  7.2%
His (H)   3	  0.7%
Ile (I)  26	  5.7%
Leu (L)  48	 10.5%
Lys (K)  41	  9.0%
Met (M)   9	  2.0%
Phe (F)  17	  3.7%
Pro (P)  17	  3.7%
Ser (S)  29	  6.4%
Thr (T)  20	  4.4%
Trp (W)   3	  0.7%
Tyr (Y)  13	  2.9%
Val (V)  28	  6.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 74

Atomic composition:

Carbon      C	      2267
Hydrogen    H	      3680
Nitrogen    N	       634
Oxygen      O	       674
Sulfur      S	        17

Formula: C2267H3680N634O674S17
Total number of atoms: 7272

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    36370
Abs 0.1% (=1 g/l)   0.711, assuming all pairs of Cys residues form cystines


Ext. coefficient    35870
Abs 0.1% (=1 g/l)   0.701, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.32
This classifies the protein as unstable.



Aliphatic index: 88.99

Grand average of hydropathicity (GRAVY): -0.374