ProtParam
User-provided sequence:
10 20 30 40 50 60
MEEERVEGSK RPFQGIIRDV KGRALCYKQD WIAGLRSGFG ILAPTTYVFF ASALPVIAFG
70 80 90 100 110 120
EQLSHDTERS LSTVETLAST ALCGVIHSLL GGQPLLILGV AEPTVLMYKY LYDFAKGRPE
130 140 150 160 170 180
LGKQLYLAWV AWVCVWTALL LFLMAIFNMA YIINRFTRIA GELFGMLIAV LFLQQTIKGM
190 200 210 220 230 240
VSEFRIPKGE DSKLEKYQFE WLYTNGLLGL IFTVGLVYTA LKSRKARSWP YGTGCCRSFV
250 260 270 280 290 300
ADYGVPLMVV VWTALSFSTP SKLPSGVPRR LVSPLPWDSV SLTHWTVIKD MGKVSPGYIF
310 320 330 340 350 360
AAFIPALMIA GLYFFDHSVV SQLAQQKEFN LKNPSAYHYD ILLLGFMVLI CGMLGLPPSN
370 380 390 400 410 420
GVLPQSPMHT KSLAVFKRQL MRRKMVMTAK ESIRQKATSS QVYEDMEQVF IEMDKSPLAE
430 440 450 460 470 480
THTTLINELQ DLKEAVMKKS DDDGDTGEES GFDPEKHVDA YLPVRVNEQR VSNLLQSLLV
490 500 510 520 530 540
IGAVFALPVI KLIPTSLLWG YFAYMAIDSL PDNQFFERTV LLFVPPTRRF KVLEGAHASF
550 560 570 580 590 600
VEKVPHKSIA AFTLFQILYF GLCYGVTWIP VAGIMFPVLF FLLVAIRQYL LPKLFKPAYL
610 620 630 640 650 660
RELDAAEYEE IPGTPRNPLE LSFRSNNSAR GVQECDAEIL DELTTSRGEL KVRTLGHNED
670 680
KGHQIYPKEI VEVGDGDMSS SRE
References and
documentation are available.
Number of amino acids: 683
Molecular weight: 76735.54
Theoretical pI: 6.88
Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 68
Atomic composition:
Carbon C 3528
Hydrogen H 5494
Nitrogen N 888
Oxygen O 966
Sulfur S 29
Formula: C
3528H
5494N
888O
966S
29
Total number of atoms: 10905
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 99740
Abs 0.1% (=1 g/l) 1.300, assuming all pairs of Cys residues form cystines
Ext. coefficient 99240
Abs 0.1% (=1 g/l) 1.293, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.87
This classifies the protein as unstable.
Aliphatic index: 100.19
Grand average of hydropathicity (GRAVY): 0.142