ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEEFTLDRNS NVGNLIALAV LNKFDELARH GKPIIRANGV REWTTLAGVV IQKKMENEFI 

        70         80         90        100        110        120 
CVCLATGVKC TPAGIIKNEQ LGSVLHDCHA EILALRCFNR LLLEHCILIK ESKKDTWLLE 

       130        140        150        160        170        180 
VADNGKFTLN SNLLIHLYVS ECPCGDASME LLASRLENNK PWNLTVDSEK LMRGRADFGL 

       190        200        210        220        230        240 
LGIVRTKPGR PDAPVSWSKS CTDKLAAKQY LSILNSQTSL ICEPIYLSCV VLYKKVIVKS 

       250        260        270        280        290        300 
AIDRAFGPFG RCAPLAEFGE KDNPYYFHPF TVLETDENFL YSRPLNQAEK TATSTNVLIW 

       310        320        330        340        350        360 
IGDKMQCTQV IHNGIKAGTK AKDVEKSQTL ICRKSMMNLL HQLSQSLTNE KNYYEWKKLN 

       370        380 
IKRCQQKQIL RNILKNWIPN GGNEFQWI 

References and documentation are available.

Number of amino acids: 388

Molecular weight: 43937.06

Theoretical pI: 8.85

Amino acid composition: 
Ala (A)  24	  6.2%
Arg (R)  17	  4.4%
Asn (N)  28	  7.2%
Asp (D)  15	  3.9%
Cys (C)  15	  3.9%
Gln (Q)  14	  3.6%
Glu (E)  25	  6.4%
Gly (G)  21	  5.4%
His (H)   8	  2.1%
Ile (I)  27	  7.0%
Leu (L)  47	 12.1%
Lys (K)  33	  8.5%
Met (M)   7	  1.8%
Phe (F)  13	  3.4%
Pro (P)  14	  3.6%
Ser (S)  22	  5.7%
Thr (T)  20	  5.2%
Trp (W)   8	  2.1%
Tyr (Y)   9	  2.3%
Val (V)  21	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 40
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1960
Hydrogen    H	      3138
Nitrogen    N	       538
Oxygen      O	       562
Sulfur      S	        22

Formula: C1960H3138N538O562S22
Total number of atoms: 6220

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    58285
Abs 0.1% (=1 g/l)   1.327, assuming all pairs of Cys residues form cystines


Ext. coefficient    57410
Abs 0.1% (=1 g/l)   1.307, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 28.67
This classifies the protein as stable.



Aliphatic index: 96.26

Grand average of hydropathicity (GRAVY): -0.207