ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEEGLLRHEN DRDDRRITAC VILSTFVAVC SSFSYGCANG YTSGAETAIM KELDLSMAQF 

        70         80         90        100        110        120 
SAFGSFLNLG GAVGALFSGQ LAVILGRRRT LWACDLFCIF GWLSIAFAKN VLWLDLGRIS 

       130        140        150        160        170        180 
LGIGVGLTSY VVPVYIAEIT PKHVRGAFSA STLLLQNSGI SLIYFFGTVI NWRVLAVIGA 

       190        200        210        220        230        240 
LPCFIPVIGI YFIPESPRWL AKIGSVKEVE NSLHRLRGKD ADVSDEAAEI QVMTKMLEED 

       250        260        270        280        290        300 
SKSSFCDMFQ KKYRRTLVVG IGLMLIQQLS GASGITYYSN AIFRKAGFSE RLGSMIFGVF 

       310        320        330        340        350        360 
VIPKALVGLI LVDRWGRRPL LLASAVGMSI GSLLIGVSFT LQEMNLFPEF IPVFVFINIL 

       370        380        390        400        410        420 
VYFGFFAIGI GGLPWIIMSE IFPINIKVSA GSIVALTSWT TGWFVSYGFN FMFEWSAQGT 

       430        440        450 
FYIFAMVGGL SLLFIWMLVP ETKGQSLEEL QASLTGTT 

References and documentation are available.

Number of amino acids: 458

Molecular weight: 50299.06

Theoretical pI: 7.54

Amino acid composition: 
Ala (A)  34	  7.4%
Arg (R)  20	  4.4%
Asn (N)  12	  2.6%
Asp (D)  12	  2.6%
Cys (C)   7	  1.5%
Gln (Q)  11	  2.4%
Glu (E)  21	  4.6%
Gly (G)  46	 10.0%
His (H)   3	  0.7%
Ile (I)  42	  9.2%
Leu (L)  53	 11.6%
Lys (K)  14	  3.1%
Met (M)  14	  3.1%
Phe (F)  36	  7.9%
Pro (P)  13	  2.8%
Ser (S)  41	  9.0%
Thr (T)  21	  4.6%
Trp (W)  11	  2.4%
Tyr (Y)  12	  2.6%
Val (V)  35	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      2333
Hydrogen    H	      3612
Nitrogen    N	       572
Oxygen      O	       622
Sulfur      S	        21

Formula: C2333H3612N572O622S21
Total number of atoms: 7160

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    78755
Abs 0.1% (=1 g/l)   1.566, assuming all pairs of Cys residues form cystines


Ext. coefficient    78380
Abs 0.1% (=1 g/l)   1.558, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.51
This classifies the protein as stable.



Aliphatic index: 110.48

Grand average of hydropathicity (GRAVY): 0.614