ProtParam
User-provided sequence:
10 20 30 40 50 60
MEEKEGIKDS SLLEKSNVPE SINEDISKTT DVDLNSDGKK DNDTSAKDGT PKVEEKVNKS
70 80 90 100 110 120
SGIDEDEVVT PAEDAKEEEE EHPPLPARRK SEEEPSKENP ILQELKDAFP NLEEKYIKAV
130 140 150 160 170 180
IIASQGVLSP AFNALLFLSD PESGKDIELP TQPVRKNPEA PARRRQTQLE QDELLARQLD
190 200 210 220 230 240
EQFNSSHSRR RNRDRATRSM HEQRRRRHNP NEREQHHEDS EEEDSWSQFV EKDLPELTDR
250 260 270 280 290 300
AGRSLQDTAN KVSNWISDAY RRNFASGNEQ NDNQHGHQDQ QEWEPEIVDL SQGGKNSRPQ
310 320 330 340 350 360
QPERRRFNSF GVQVGDDSLE SHGITLHNED GFEDDEDVPP QLPTRTKSGE STGKVVAETT
370 380 390 400 410
YIDTPDTETK KKWQPLPPEP LDTTPTKVNA VSRNKKNPDE DEFLINSDDE M
References and
documentation are available.
Number of amino acids: 411
Molecular weight: 46869.67
Theoretical pI: 4.70
Total number of negatively charged residues (Asp + Glu): 93
Total number of positively charged residues (Arg + Lys): 57
Atomic composition:
Carbon C 1992
Hydrogen H 3149
Nitrogen N 597
Oxygen O 707
Sulfur S 3
Formula: C
1992H
3149N
597O
707S
3
Total number of atoms: 6448
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 26470
Abs 0.1% (=1 g/l) 0.565
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 71.19
This classifies the protein as unstable.
Aliphatic index: 55.99
Grand average of hydropathicity (GRAVY): -1.373