ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEELTQILKN NRTDDLTWFC SLSESELDLL ISLKKLAIQR AKISGHQELA DKFDLKLLRS 

        70         80         90        100        110        120 
LGLVLMEHAR KRVQNDTSLA PSVVHQLRLL DNCNLLKTHV DDAVDIEEIL TQICDNKSKK 


KARKRRR 

References and documentation are available.

Number of amino acids: 127

Molecular weight: 14688.08

Theoretical pI: 9.07

Amino acid composition: 
Ala (A)   7	  5.5%
Arg (R)  10	  7.9%
Asn (N)   6	  4.7%
Asp (D)  11	  8.7%
Cys (C)   3	  2.4%
Gln (Q)   6	  4.7%
Glu (E)   8	  6.3%
Gly (G)   2	  1.6%
His (H)   4	  3.1%
Ile (I)   7	  5.5%
Leu (L)  23	 18.1%
Lys (K)  13	 10.2%
Met (M)   2	  1.6%
Phe (F)   2	  1.6%
Pro (P)   1	  0.8%
Ser (S)   9	  7.1%
Thr (T)   6	  4.7%
Trp (W)   1	  0.8%
Tyr (Y)   0	  0.0%
Val (V)   6	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       639
Hydrogen    H	      1081
Nitrogen    N	       191
Oxygen      O	       193
Sulfur      S	         5

Formula: C639H1081N191O193S5
Total number of atoms: 2109

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     5625
Abs 0.1% (=1 g/l)   0.383, assuming all pairs of Cys residues form cystines


Ext. coefficient     5500
Abs 0.1% (=1 g/l)   0.374, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.41
This classifies the protein as unstable.



Aliphatic index: 111.34

Grand average of hydropathicity (GRAVY): -0.457