ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEENQLTCLI VGETPAAQFL GWRLSLSNSF IILVSQYVSS DELVAWKSTK LGANFYTPNI 

        70         80         90        100        110        120 
LTKDIKELHH KLKHNGANTY SIDIVLVSAI SLQSFETTCR LLSDYTNDNT TVLISTDFGC 

       130        140        150        160        170        180 
ELEPIAISYF GGKCKCVISI SCEVECRQLS LGSYALVNDD QCVITLGLTY CDANFEASPT 

       190        200        210        220        230        240 
ILENTKAASL ELQGIKGSNV RRFLLGLTVA KWMKSKLILD PKQMALKMWE LLIPKISLNI 

       250        260        270        280        290        300 
LSIIYEQFDY EKMLENKSTE IIFKDLVKEL LGICFAQCGS KIARFLLVKS QGEEEINFGK 

       310        320        330        340        350        360 
IVEYCKGKKL QLINSTANEH PEYLSLPFEP YCFYHRFEYP AQILLHQPII LAKKYGVSCS 

       370        380        390        400        410        420 
NLNFLYGFYT RLLTLSGLSI NGGRCEHALS MLDSRIGGGI NVASGINSGQ DFTDGDNEDQ 

       430        440        450        460        470        480 
DKGKNRVDKN VKEGSFISLT QRFTMTSPLG VNDPALPADL EKLYLGAEYI SNCDANTSGG 

       490        500        510        520        530        540 
QKRVKSPTKA DTGYDDKHLP DDTIHTFEDE YLADEDDFSC LGIDKRSSTK PTKPLEKFGV 

       550        560        570        580        590        600 
VAVPHFIRRF SIKRSSKDKS NDMKRPYTTS SLELQLRSNH FMFAKEYQDL HRQLYYEVKP 

       610        620        630        640        650        660 
RTQSELDARR RNYSELESQM WKIKHRFNIH RGALPRPRTN PYELLLDHID VLNRGNTGDI 

       670        680        690        700        710        720 
LRFTTSRYGG VDTYDSILRD QSTIMELLDK RCAYSPPVLF DNEAKERDHD RDCYQDYNHD 


HRSH 

References and documentation are available.

Number of amino acids: 724

Molecular weight: 82327.64

Theoretical pI: 6.21

Amino acid composition: 
Ala (A)  33	  4.6%
Arg (R)  36	  5.0%
Asn (N)  37	  5.1%
Asp (D)  49	  6.8%
Cys (C)  19	  2.6%
Gln (Q)  25	  3.5%
Glu (E)  46	  6.4%
Gly (G)  42	  5.8%
His (H)  19	  2.6%
Ile (I)  48	  6.6%
Leu (L)  83	 11.5%
Lys (K)  51	  7.0%
Met (M)  11	  1.5%
Phe (F)  31	  4.3%
Pro (P)  26	  3.6%
Ser (S)  61	  8.4%
Thr (T)  41	  5.7%
Trp (W)   5	  0.7%
Tyr (Y)  32	  4.4%
Val (V)  29	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 95
Total number of positively charged residues (Arg + Lys): 87

Atomic composition:

Carbon      C	      3660
Hydrogen    H	      5746
Nitrogen    N	       988
Oxygen      O	      1111
Sulfur      S	        30

Formula: C3660H5746N988O1111S30
Total number of atoms: 11535

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    76305
Abs 0.1% (=1 g/l)   0.927, assuming all pairs of Cys residues form cystines


Ext. coefficient    75180
Abs 0.1% (=1 g/l)   0.913, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.49
This classifies the protein as stable.



Aliphatic index: 86.74

Grand average of hydropathicity (GRAVY): -0.394