ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEENSADIEN RPVAAFRPAV SVPMPVLPQD GEVFVGPGGK KDAQKIGLGL SKLSSKRKDD 

        70         80         90        100        110        120 
IQMAKKYAMD ISIKQILLRQ QKQQQENQQR QQMYSQALSI MSRIYVGSIS FEIREDMLRR 

       130        140        150        160        170        180 
AFDPFGPIKS INMSWDPATG HHKTFAFVEY EVPEAALLAQ ESMNGQMLGG RNLKVNSMMF 

       190        200        210        220        230        240 
QEMRLPQNMP QAQPIIDMVQ KDAKKYFRVY VSSVHPDLSE TDLKSVFEAF GEIVKCQLAR 

       250        260        270        280        290        300 
APTGRGHRGF GYLEFNNLTS QSEAIAGMNM FDLGGQYLRV GKCVTPPDAL TYLQPASVSA 

       310        320        330        340        350        360 
IPASVSVACA AITAKVMAAE AAAGSSPKTP SESGGSRAAS PAPRAQSPAT PSSSLPTDIE 

       370        380        390        400        410        420 
NKAVISSPKK EPEEIEVPPL PPSAPDVVKD EPMEIEEEEE YTIPEEKPKP VAIVPPPGLA 

       430        440        450        460        470        480 
IPSLVAPPGL IAPTEIGIVV PNPSFLAQQQ QKIEEKIEEE EEARTERVKL STSQRKKMKR 

       490        500        510        520        530        540 
EKLNQMTFEE KMAQVLGQQK AVQNQRMADP VTFGALDDTV AWKDPSNEDQ TSEDGKMLAI 

       550        560        570        580        590        600 
MGPGRGGDNV ASMALALMDG GSSLMLANNA KAKEAAAALA LEPKKKKKVK EGKKIQPKLN 

       610        620        630        640        650        660 
TAQALAAAAK AGEMSDALKN EVMNSEDASL ASQEGLEIRG NDARHLLMTK LMRTNRSNVI 

       670        680        690        700        710        720 
VLRNMVTPQD IDEFLEGEIR EECGKYGNVI DVVIANFASS GLVKIFVKYS DSMQVDRAKA 

       730        740 
ALDGRFFGGN TVKAEAYDQI LFDHADYTG 

References and documentation are available.

Number of amino acids: 749

Molecular weight: 81616.28

Theoretical pI: 5.55

Amino acid composition: 
Ala (A)  83	 11.1%
Arg (R)  32	  4.3%
Asn (N)  29	  3.9%
Asp (D)  38	  5.1%
Cys (C)   4	  0.5%
Gln (Q)  44	  5.9%
Glu (E)  61	  8.1%
Gly (G)  48	  6.4%
His (H)   6	  0.8%
Ile (I)  40	  5.3%
Leu (L)  52	  6.9%
Lys (K)  57	  7.6%
Met (M)  34	  4.5%
Phe (F)  23	  3.1%
Pro (P)  52	  6.9%
Ser (S)  56	  7.5%
Thr (T)  25	  3.3%
Trp (W)   2	  0.3%
Tyr (Y)  14	  1.9%
Val (V)  49	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 99
Total number of positively charged residues (Arg + Lys): 89

Atomic composition:

Carbon      C	      3570
Hydrogen    H	      5765
Nitrogen    N	       989
Oxygen      O	      1116
Sulfur      S	        38

Formula: C3570H5765N989O1116S38
Total number of atoms: 11478

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32110
Abs 0.1% (=1 g/l)   0.393, assuming all pairs of Cys residues form cystines


Ext. coefficient    31860
Abs 0.1% (=1 g/l)   0.390, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 54.85
This classifies the protein as unstable.



Aliphatic index: 77.96

Grand average of hydropathicity (GRAVY): -0.401