ProtParam
User-provided sequence:
10 20 30 40 50 60
MEERKLLRAV KSTNPLVYLD ISIGKEDAGR MIIELRKDVV PKTAENFRAL CTGECGIGTL
70 80 90 100 110 120
GKPLHYKGTK FHKIKRVFVV QSGDVVKNDG SSGESIYGPV FDDENFELSH NEEGVVSMAN
130 140 150 160 170 180
YGKPNSNNSQ FFISAAGCEN LNGTNVVVGR VLRGLGIVAE MEQNCTDEGD PTAPIVIRDC
190 200 210 220 230 240
GEIAHNEDWG IECNDETTDK LPAYPQDWPR KLDKFTGDGA VELLTGIRQS GNHFYQLGRY
250 260 270 280 290 300
HEARAKYRKA NRYYHYLSRQ FGWQQLNPLK KHLVDEDLLK VDGFSVVNNI NAAAVDLKVG
310 320 330 340 350 360
NYTSAREVCN EAIRLDPKCS KAFYRRAQAQ RGLRNYEEAI NDLKTAHNLL PENKQILNEL
370 380
NSTKQLLAQY NRQQRNALKN LFA
References and
documentation are available.
Number of amino acids: 383
Molecular weight: 43115.62
Theoretical pI: 7.58
Total number of negatively charged residues (Asp + Glu): 50
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 1893
Hydrogen H 2989
Nitrogen N 551
Oxygen O 579
Sulfur S 12
Formula: C
1893H
2989N
551O
579S
12
Total number of atoms: 6024
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 39350
Abs 0.1% (=1 g/l) 0.913, assuming all pairs of Cys residues form cystines
Ext. coefficient 38850
Abs 0.1% (=1 g/l) 0.901, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.05
This classifies the protein as stable.
Aliphatic index: 80.97
Grand average of hydropathicity (GRAVY): -0.592