ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEESKRNLLD EEAKASLDIW RYVFGFADIA AAKCAIDLKI PEAIENHPSS QPVTLSELSS 

        70         80         90        100        110        120 
AVSASPSHLR RIMRFLVHQG LFKEVPTKDG LATGYTNTPL SRRMMITKLH GKDLWAFAQD 

       130        140        150        160        170        180 
NLCHSQLINE AMACDARRVV PRVAGACQGL FDGVATVVDV GGGTGETMGI LVKEFPWIKG 

       190        200        210        220        230        240 
FNFDLPHVIE VAQVLDGVEN VEGDMFDSIP ASDAVIIKWV LHDWGDKDCI KILKNCKEAV 

       250        260        270        280        290        300 
LPNIGKVLIV ECVIGEKKNT MIAEERDDKL EHVRLQLDMV MMVHTSTGKE RTLKEWDFVL 

       310        320 
TEAGFARYEV RDFDDVQSLI IAYRS 

References and documentation are available.

Number of amino acids: 325

Molecular weight: 36259.79

Theoretical pI: 5.28

Amino acid composition: 
Ala (A)  27	  8.3%
Arg (R)  16	  4.9%
Asn (N)  10	  3.1%
Asp (D)  25	  7.7%
Cys (C)   7	  2.2%
Gln (Q)   8	  2.5%
Glu (E)  24	  7.4%
Gly (G)  21	  6.5%
His (H)   9	  2.8%
Ile (I)  22	  6.8%
Leu (L)  29	  8.9%
Lys (K)  21	  6.5%
Met (M)  11	  3.4%
Phe (F)  13	  4.0%
Pro (P)  11	  3.4%
Ser (S)  17	  5.2%
Thr (T)  14	  4.3%
Trp (W)   6	  1.8%
Tyr (Y)   4	  1.2%
Val (V)  30	  9.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1612
Hydrogen    H	      2562
Nitrogen    N	       436
Oxygen      O	       477
Sulfur      S	        18

Formula: C1612H2562N436O477S18
Total number of atoms: 5105

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39335
Abs 0.1% (=1 g/l)   1.085, assuming all pairs of Cys residues form cystines


Ext. coefficient    38960
Abs 0.1% (=1 g/l)   1.074, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.72
This classifies the protein as stable.



Aliphatic index: 96.28

Grand average of hydropathicity (GRAVY): -0.057