ProtParam
User-provided sequence:
10 20 30 40 50 60
MEESKTKRKE DRIDLKNTPP QKKSKRDSTN DETARTSLRS IMPRGYKMME NMGYKEGETL
70 80 90 100 110 120
GSNESALKEP IKVEINTKRR GIRAEKPDPS TMNDMQMSEQ RFIKRESEMK NNKRLKKIWY
130 140 150 160 170 180
RIQKVAFEMM GDSDLYNPGE DPRDFNVLWR SYVMQLNEEV AKNDLNNHSN NDNEDKNNMI
190 200 210 220 230 240
PMVNESLEAS PAIKGEKFGR PSTLIDCDTS IIGSRITKDT ELAELEELSI EKRITKLNIF
250 260 270
LRSEKYYCFF CGIKYKDEGD LYEHCPGVNE DDHK
References and
documentation are available.
Number of amino acids: 274
Molecular weight: 32004.15
Theoretical pI: 6.11
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 49
Atomic composition:
Carbon C 1375
Hydrogen H 2212
Nitrogen N 396
Oxygen O 446
Sulfur S 18
Formula: C
1375H
2212N
396O
446S
18
Total number of atoms: 4447
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 24660
Abs 0.1% (=1 g/l) 0.771, assuming all pairs of Cys residues form cystines
Ext. coefficient 24410
Abs 0.1% (=1 g/l) 0.763, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.46
This classifies the protein as unstable.
Aliphatic index: 60.15
Grand average of hydropathicity (GRAVY): -1.104