ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFISADYSS SDGSDTESES SNKSEVQIEY TEKTCIQKAD STDLPAIPDS IILKYHIPPN 

        70         80         90        100        110        120 
LQKYEHQDMN MSRFWRSFTY FEWRPTPAIH RQLQKIICKY KETFMKQEYT NPYQLVDFDP 

       130        140        150        160        170        180 
LFISHLGAPK PLHVSLTRSL LFETEEQRHV FIQEMRNGLR NNEITPFKLQ ICSYPKLYIS 

       190        200        210        220        230        240 
ERANTLYLGL PVSECPNKAQ ISPFKTIIAE ALQKSGISNY QDLIVSRQNL HVSIAIASNP 

       250        260        270        280        290 
SKATLKRYQQ LNETMGALLL LNNDFAYKLE FLVNSIYCDE NRHSIRIPFN 

References and documentation are available.

Number of amino acids: 290

Molecular weight: 33694.36

Theoretical pI: 6.46

Amino acid composition: 
Ala (A)  14	  4.8%
Arg (R)  13	  4.5%
Asn (N)  18	  6.2%
Asp (D)  12	  4.1%
Cys (C)   5	  1.7%
Gln (Q)  17	  5.9%
Glu (E)  21	  7.2%
Gly (G)   6	  2.1%
His (H)   8	  2.8%
Ile (I)  25	  8.6%
Leu (L)  28	  9.7%
Lys (K)  18	  6.2%
Met (M)   6	  2.1%
Phe (F)  14	  4.8%
Pro (P)  17	  5.9%
Ser (S)  28	  9.7%
Thr (T)  15	  5.2%
Trp (W)   2	  0.7%
Tyr (Y)  15	  5.2%
Val (V)   8	  2.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 31

Atomic composition:

Carbon      C	      1513
Hydrogen    H	      2348
Nitrogen    N	       400
Oxygen      O	       450
Sulfur      S	        11

Formula: C1513H2348N400O450S11
Total number of atoms: 4722

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    33600
Abs 0.1% (=1 g/l)   0.997, assuming all pairs of Cys residues form cystines


Ext. coefficient    33350
Abs 0.1% (=1 g/l)   0.990, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.71
This classifies the protein as unstable.



Aliphatic index: 84.10

Grand average of hydropathicity (GRAVY): -0.467