ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFKRDNTVR FYGDEKQTIE VGEKRVPLFK STTAPFMKQE VLPKKSKTRL KIPRFGRFKV 

        70         80         90        100        110        120 
FPENFEIERD KILDPGGDAV LQWNRVFLFW CLVALYVDPL FFFLSSVKRI GRSSCMTTDL 

       130        140        150        160        170        180 
KLGIVITFFR TLADLFYVLH IVIKFRTAYV SRTSRVFGRG ELVKDPKLIA RRYLRSDFIV 

       190        200        210        220        230        240 
DLIACLPLPQ IVSWFILPSI RSSHSDHTTN ALVLIVLVQY IPRLYLIFPL SAEIIKATGV 

       250        260        270        280        290        300 
VTTTAWAGAA YNLLQYMLAS HILGSAWYLL SIERQATCWK AECHKESVPL QCVTDFFDCG 

       310        320        330        340        350        360 
TLHRDDRNNW QNTTVVFSNC DPSNNIQFTF GIFADALTKN VVSSPFLEKY LYCLWFGLQN 

       370        380        390        400        410        420 
LSSYGQNLST STSVLETMFA ILVAIFGLVL FALLIGNMQT YLQSITVRLE EWRLKRRDTE 

       430        440        450        460        470        480 
EWMGHRLLPQ NLRERVRRFV QYKWLATRGV DEETILHSLP ADLRRDIQRH LCLDLVRRVP 

       490        500        510        520        530        540 
LFAQMDDQLL DAICERLASS LSTQGNYIVR EGDPVTEMLF IIRGKLESST TNGGRTGFFN 

       550        560        570        580        590        600 
SITLRPGDFC GEELLAWALL PKSTVNLPSS TRTVRALEEV EAFALQAGDL KFVANQFRRL 

       610        620        630        640        650        660 
HSKKLQHTFR YYSHQWRTWA ACFVQVAWRR YKRKKLAKSL SLAESFSSYD EEEAVAVAAT 

       670        680        690        700        710        720 
EEMSHEGEAQ SGAKARHHTS NVKPHFAATI LASRFAKNTR RTAHKLKDVE IPMLPKPDEP 


DFSVDD 

References and documentation are available.

Number of amino acids: 726

Molecular weight: 83454.47

Theoretical pI: 9.24

Amino acid composition: 
Ala (A)  52	  7.2%
Arg (R)  56	  7.7%
Asn (N)  23	  3.2%
Asp (D)  35	  4.8%
Cys (C)  13	  1.8%
Gln (Q)  26	  3.6%
Glu (E)  41	  5.6%
Gly (G)  31	  4.3%
His (H)  17	  2.3%
Ile (I)  37	  5.1%
Leu (L)  86	 11.8%
Lys (K)  38	  5.2%
Met (M)  11	  1.5%
Phe (F)  47	  6.5%
Pro (P)  28	  3.9%
Ser (S)  51	  7.0%
Thr (T)  48	  6.6%
Trp (W)  15	  2.1%
Tyr (Y)  20	  2.8%
Val (V)  51	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 94

Atomic composition:

Carbon      C	      3791
Hydrogen    H	      5926
Nitrogen    N	      1030
Oxygen      O	      1047
Sulfur      S	        24

Formula: C3791H5926N1030O1047S24
Total number of atoms: 11818

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   113050
Abs 0.1% (=1 g/l)   1.355, assuming all pairs of Cys residues form cystines


Ext. coefficient   112300
Abs 0.1% (=1 g/l)   1.346, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.38
This classifies the protein as unstable.



Aliphatic index: 93.61

Grand average of hydropathicity (GRAVY): -0.106