ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFQAVVMAV GGGSRMTDLT SSIPKPLLPV GNKPLIWYPL NLLERVGFEE VIVVTTKDVQ 

        70         80         90        100        110        120 
KALCAEFKMK LKPDIVCIPD EADMGTADSL RHIYPKLKTD VLVLGCDLIT DVALHEVVDL 

       130        140        150        160        170        180 
FRAYDASLAM LMRKGQESTE PVPGQKGKKK TVEQRDFIGV DSTGKRLLFM ANEADLDEEL 

       190        200        210        220        230        240 
VIKGSILQKH PRIHFQTGLV DAHLYCLKKY VVDFLMENKS ITSIRSELIP YLVRKQFSSA 

       250        260        270        280        290        300 
SSQQRQEDKE EDLKKKEPKS LDIYSFIKKD NTLTLAPYDA CWNAFRRDKW EDLSRSQVRC 

       310        320        330        340        350        360 
YVHIMKEGLC SRVSTLGLYM EANRQVPKLL SVLCPEESMI HPSAQIANKH LIGADSLIGS 

       370        380        390        400        410        420 
DTQVGEKSSI KRSVIGSSCV IRDRVTVTNC LLMNSVTVEE GSSIHGSVIC NNAVVEAGAE 

       430        440        450 
IRDCLIGSGQ RIEAKAKRMN EVIVGNDQLM EI 

References and documentation are available.

Number of amino acids: 452

Molecular weight: 50435.47

Theoretical pI: 6.54

Amino acid composition: 
Ala (A)  26	  5.8%
Arg (R)  23	  5.1%
Asn (N)  14	  3.1%
Asp (D)  29	  6.4%
Cys (C)  12	  2.7%
Gln (Q)  17	  3.8%
Glu (E)  32	  7.1%
Gly (G)  26	  5.8%
His (H)   9	  2.0%
Ile (I)  31	  6.9%
Leu (L)  46	 10.2%
Lys (K)  36	  8.0%
Met (M)  15	  3.3%
Phe (F)  11	  2.4%
Pro (P)  17	  3.8%
Ser (S)  36	  8.0%
Thr (T)  19	  4.2%
Trp (W)   3	  0.7%
Tyr (Y)  10	  2.2%
Val (V)  40	  8.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 61
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2219
Hydrogen    H	      3623
Nitrogen    N	       609
Oxygen      O	       671
Sulfur      S	        27

Formula: C2219H3623N609O671S27
Total number of atoms: 7149

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32150
Abs 0.1% (=1 g/l)   0.637, assuming all pairs of Cys residues form cystines


Ext. coefficient    31400
Abs 0.1% (=1 g/l)   0.623, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.38
This classifies the protein as stable.



Aliphatic index: 97.85

Grand average of hydropathicity (GRAVY): -0.159