ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFQGTLPFH PLEDPETLLL KFTDFVHQHS VEQCNQLSMY IIASVNSLAE AFYGSHPEKY 

        70         80         90        100        110        120 
NIVFQSVEYA AHSPAISVMH VCYLKELLRQ GQYATSLKSF NDLEVSKHIP GSILLQYCMY 

       130        140        150        160        170        180 
AAYHCLGNND LDSAKVWYFS ILYIPTTTLT SFHEEAYYSF LLLYIITTGK KFQLDSATSS 

       190        200        210        220        230        240 
NVLPLKRHMV PYEEFLDAYL KDVNTLRTVI KEHWSRFLKD NSTAFILFAL EVYPMHRLKK 

       250        260        270        280        290        300 
WRKTFSSLKL EYIAKQLAIS QDVAKEIIQK FDNKTNFTVA NGEIFLTFNA LDDVSPEMHS 

       310        320        330 
DLCQQLIEAS KNFEASIRLK SVIYSKIMAK KLNA 

References and documentation are available.

Number of amino acids: 334

Molecular weight: 38534.38

Theoretical pI: 6.50

Amino acid composition: 
Ala (A)  24	  7.2%
Arg (R)   7	  2.1%
Asn (N)  15	  4.5%
Asp (D)  14	  4.2%
Cys (C)   5	  1.5%
Gln (Q)  14	  4.2%
Glu (E)  21	  6.3%
Gly (G)   7	  2.1%
His (H)  13	  3.9%
Ile (I)  21	  6.3%
Leu (L)  40	 12.0%
Lys (K)  25	  7.5%
Met (M)   8	  2.4%
Phe (F)  21	  6.3%
Pro (P)  11	  3.3%
Ser (S)  30	  9.0%
Thr (T)  18	  5.4%
Trp (W)   3	  0.9%
Tyr (Y)  19	  5.7%
Val (V)  18	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 35
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1768
Hydrogen    H	      2710
Nitrogen    N	       438
Oxygen      O	       501
Sulfur      S	        13

Formula: C1768H2710N438O501S13
Total number of atoms: 5430

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    45060
Abs 0.1% (=1 g/l)   1.169, assuming all pairs of Cys residues form cystines


Ext. coefficient    44810
Abs 0.1% (=1 g/l)   1.163, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.08
This classifies the protein as unstable.



Aliphatic index: 94.04

Grand average of hydropathicity (GRAVY): -0.081