ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFSTSKRPA TTATAAESVH FRLLCPATRT GAIIGKGGSV IRHLQSVTGS KIRVIDDIPV 

        70         80         90        100        110        120 
PSEERVVLII APSGKKKDES NVCDSENPGS EEPKQEKGSE CAGTSGGDDE EAPSSAQMAL 

       130        140        150        160        170        180 
LRVFERIVFG DDAATVDGDE LDKGESEGLC RMIVRGNQVD YLMSKGGKMI QKIREDSGAI 

       190        200        210        220        230        240 
VRISSTDQIP PCAFPGDVVI QMNGKFSSVK KALLLVTNCL QESGAPPTWD ECPFPQPGYP 

       250        260        270        280        290        300 
PEYHSMEYHP QWDHPPPNPM PEDVGPFNRP VVEEEVAFRL LCPADKVGSL IGKGGAVVRA 

       310        320        330        340        350        360 
LQNESGASIK VSDPTHDSEE RIIVISAREN LERRHSLAQD GVMRVHNRIV EIGFEPSAAV 

       370        380        390        400        410        420 
VARLLVHSPY IGRLLGKGGH LISEMRRATG ASIRVFAKDQ ATKYESQHDE IVQVIGNLKT 

       430        440        450        460        470        480 
VQDALFQILC RLREAMFPGR LPFQGMGGPP PPFMGPYPEP PPPFGPRQYP ASPDRYHSPV 

       490        500        510        520        530        540 
GPFHERHCHG PGFDRPPGPG FDRPPSPMSW TPQPGIDGHP GGMVPPDVNH GFALRNEPIG 

       550        560        570        580        590        600 
SENPVMTSAN VEIVIPQAYL GHVYGENCSN LNYIKQVSGA NVVVHDPKAG TTEGLVVVSG 

       610        620 
TSDQAHFAQS LLHAFILCGQ S 

References and documentation are available.

Number of amino acids: 621

Molecular weight: 67115.01

Theoretical pI: 5.74

Amino acid composition: 
Ala (A)  42	  6.8%
Arg (R)  35	  5.6%
Asn (N)  19	  3.1%
Asp (D)  32	  5.2%
Cys (C)  12	  1.9%
Gln (Q)  25	  4.0%
Glu (E)  43	  6.9%
Gly (G)  61	  9.8%
His (H)  21	  3.4%
Ile (I)  35	  5.6%
Leu (L)  36	  5.8%
Lys (K)  24	  3.9%
Met (M)  16	  2.6%
Phe (F)  22	  3.5%
Pro (P)  63	 10.1%
Ser (S)  48	  7.7%
Thr (T)  21	  3.4%
Trp (W)   3	  0.5%
Tyr (Y)  12	  1.9%
Val (V)  51	  8.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 59

Atomic composition:

Carbon      C	      2951
Hydrogen    H	      4633
Nitrogen    N	       839
Oxygen      O	       897
Sulfur      S	        28

Formula: C2951H4633N839O897S28
Total number of atoms: 9348

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35130
Abs 0.1% (=1 g/l)   0.523, assuming all pairs of Cys residues form cystines


Ext. coefficient    34380
Abs 0.1% (=1 g/l)   0.512, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.65
This classifies the protein as unstable.



Aliphatic index: 75.17

Grand average of hydropathicity (GRAVY): -0.363