ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFWGIEIKP GKPFKVIQKD GFMVHASQVT LGDVEKVKKD ETFAVYVKIG DDENGFMIGN 

        70         80         90        100        110        120 
LSQKFPQFSI DLYLGHEFEI SHNSTSSVYL IGYRTFDAFD ELDEEIDSDS ELDEYMEQQI 

       130        140        150        160        170        180 
AALPQNEINP EEDDESDSDE MGLDEDDDSS DEEDVEAEAP LKVAPPSKKM PNGAFEIAKG 

       190        200 
GKKNKSSGGK KRCPFPCGPS CKK 

References and documentation are available.

Number of amino acids: 203

Molecular weight: 22651.04

Theoretical pI: 4.34

Amino acid composition: 
Ala (A)  10	  4.9%
Arg (R)   2	  1.0%
Asn (N)   7	  3.4%
Asp (D)  22	 10.8%
Cys (C)   3	  1.5%
Gln (Q)   7	  3.4%
Glu (E)  24	 11.8%
Gly (G)  16	  7.9%
His (H)   3	  1.5%
Ile (I)  12	  5.9%
Leu (L)  10	  4.9%
Lys (K)  20	  9.9%
Met (M)   6	  3.0%
Phe (F)  12	  5.9%
Pro (P)  12	  5.9%
Ser (S)  17	  8.4%
Thr (T)   4	  2.0%
Trp (W)   1	  0.5%
Tyr (Y)   5	  2.5%
Val (V)  10	  4.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	       995
Hydrogen    H	      1522
Nitrogen    N	       250
Oxygen      O	       336
Sulfur      S	         9

Formula: C995H1522N250O336S9
Total number of atoms: 3112

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    13075
Abs 0.1% (=1 g/l)   0.577, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.572, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.31
This classifies the protein as unstable.



Aliphatic index: 61.48

Grand average of hydropathicity (GRAVY): -0.746