ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFWGIEVKS GKPVTVTPEE GILIHVSQAS LGECKNKKGE FVPLHVKVGN QNLVLGTLST 

        70         80         90        100        110        120 
ENIPQLFCDL VFDKEFELSH TWGKGSVYFV GYKTPNIEPQ GYSEEEEEEE EEVPAGNAAK 

       130        140        150        160        170        180 
AVAKPKAKPA EVKPAVDDEE DESDSDGMDE DDSDGEDSEE EEPTPKKPAS SKKRANETTP 

       190        200        210        220        230        240 
KAPVSAKKAK VAVTPQKTDE KKKGGKAANQ SPKSASQVSC GSCKKTFNSG NALESHNKAK 


HAAAK 

References and documentation are available.

Number of amino acids: 245

Molecular weight: 26372.23

Theoretical pI: 5.12

Amino acid composition: 
Ala (A)  23	  9.4%
Arg (R)   1	  0.4%
Asn (N)  11	  4.5%
Asp (D)  13	  5.3%
Cys (C)   4	  1.6%
Gln (Q)   7	  2.9%
Glu (E)  32	 13.1%
Gly (G)  18	  7.3%
His (H)   5	  2.0%
Ile (I)   5	  2.0%
Leu (L)  10	  4.1%
Lys (K)  33	 13.5%
Met (M)   2	  0.8%
Phe (F)   7	  2.9%
Pro (P)  17	  6.9%
Ser (S)  21	  8.6%
Thr (T)  12	  4.9%
Trp (W)   2	  0.8%
Tyr (Y)   3	  1.2%
Val (V)  19	  7.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1145
Hydrogen    H	      1817
Nitrogen    N	       311
Oxygen      O	       390
Sulfur      S	         6

Formula: C1145H1817N311O390S6
Total number of atoms: 3669

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15720
Abs 0.1% (=1 g/l)   0.596, assuming all pairs of Cys residues form cystines


Ext. coefficient    15470
Abs 0.1% (=1 g/l)   0.587, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.84
This classifies the protein as unstable.



Aliphatic index: 55.76

Grand average of hydropathicity (GRAVY): -0.898