ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEFWGVAVTP KNATKVTPEE DSLVHISQAS LDCTVKSGES VVLSVTVGGA KLVIGTLSQD 

        70         80         90        100        110        120 
KFPQISFDLV FDKEFELSHS GTKANVHFIG YKSPNIEQDD FTSSDDEDVP EAVPAPAPTA 

       130        140        150        160        170        180 
VTANGNAGAA VVKADTKPKA KPAEVKPAEE KPESDEEDES DDEDESEEDD DSEKGMDVDE 

       190        200        210        220        230        240 
DDSDDDEEED SEDEEEEETP KKPEPINKKR PNESVSKTPV SGKKAKPAAA PASTPQKTEE 

       250        260        270        280        290        300 
KKKGGHTATP HPAKKGGKSP VNANQSPKSG GQSSGGNNNK KPFNSGKQFG GSNNKGSNKG 


KGKGRA 

References and documentation are available.

Number of amino acids: 306

Molecular weight: 32347.96

Theoretical pI: 4.68

Amino acid composition: 
Ala (A)  26	  8.5%
Arg (R)   2	  0.7%
Asn (N)  16	  5.2%
Asp (D)  28	  9.2%
Cys (C)   1	  0.3%
Gln (Q)   8	  2.6%
Glu (E)  35	 11.4%
Gly (G)  26	  8.5%
His (H)   5	  1.6%
Ile (I)   6	  2.0%
Leu (L)   7	  2.3%
Lys (K)  38	 12.4%
Met (M)   2	  0.7%
Phe (F)   9	  2.9%
Pro (P)  25	  8.2%
Ser (S)  31	 10.1%
Thr (T)  17	  5.6%
Trp (W)   1	  0.3%
Tyr (Y)   1	  0.3%
Val (V)  22	  7.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1379
Hydrogen    H	      2183
Nitrogen    N	       385
Oxygen      O	       506
Sulfur      S	         3

Formula: C1379H2183N385O506S3
Total number of atoms: 4456

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     6990
Abs 0.1% (=1 g/l)   0.216, assuming all pairs of Cys residues form cystines


Ext. coefficient     6990
Abs 0.1% (=1 g/l)   0.216, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 61.35
This classifies the protein as unstable.



Aliphatic index: 45.92

Grand average of hydropathicity (GRAVY): -1.120