ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEGENSSIEK GLLLIRKEES ANTTPFLVFT TFIIVSASFS FGVALGHTAG TMASIMEDLD 

        70         80         90        100        110        120 
LSITQFSVFG SLLTFGGMIG ALFSATIADS FGCKMTLWIT EVFCISGWLA IALAKNIIWL 

       130        140        150        160        170        180 
DLGRFFVGIG VGLLSYVVPV YIAEITPKTV RGTFTFSNQL LQNCGVATAY YLGNFMSWRI 

       190        200        210        220        230        240 
IALIGILPCL IQLVGLFFVP ESPRWLAKEG RDEECEVVLQ KLRGDEADIV KETQEILISV 

       250        260        270        280        290        300 
EASANISMRS LFKKKYTHQL TIGIGLMLLQ QLSGSAGLGY YTGSVFDLAG FPSRIGMTVL 

       310        320 
SIVVVPKAIL GLILVERWGR RPLLMVM 

References and documentation are available.

Number of amino acids: 327

Molecular weight: 35713.16

Theoretical pI: 5.82

Amino acid composition: 
Ala (A)  22	  6.7%
Arg (R)  12	  3.7%
Asn (N)   7	  2.1%
Asp (D)   8	  2.4%
Cys (C)   5	  1.5%
Gln (Q)   9	  2.8%
Glu (E)  18	  5.5%
Gly (G)  32	  9.8%
His (H)   2	  0.6%
Ile (I)  31	  9.5%
Leu (L)  43	 13.1%
Lys (K)  12	  3.7%
Met (M)  11	  3.4%
Phe (F)  21	  6.4%
Pro (P)   9	  2.8%
Ser (S)  26	  8.0%
Thr (T)  21	  6.4%
Trp (W)   6	  1.8%
Tyr (Y)   7	  2.1%
Val (V)  25	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      1645
Hydrogen    H	      2605
Nitrogen    N	       401
Oxygen      O	       450
Sulfur      S	        16

Formula: C1645H2605N401O450S16
Total number of atoms: 5117

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43680
Abs 0.1% (=1 g/l)   1.223, assuming all pairs of Cys residues form cystines


Ext. coefficient    43430
Abs 0.1% (=1 g/l)   1.216, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.96
This classifies the protein as unstable.



Aliphatic index: 117.16

Grand average of hydropathicity (GRAVY): 0.637