ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEGEQSPQGE KSLQRKQFVR PPLDLWQDLK DGVRYVIFDC RANLIVMPLL ILFESMLCKI 

        70         80         90        100        110        120 
IIKKVAYTEI DYKAYMEQIE MIQLDGMLDY SQVSGGTGPL VYPAGHVLIY KMMYWLTEGM 

       130        140        150        160        170        180 
DHVERGQVFF RYLYLLTLAL QMACYYLLHL PPWCVVLACL SKRLHSIYVL RLFNDCFTTL 

       190        200        210        220        230        240 
FMVVTVLGAI VASRCHQRPK LKKSLALVIS ATYSMAVSIK MNALLYFPAM MISLFILNDA 

       250        260        270        280        290        300 
NVILTLLDLV AMIAWQVAVA VPFLRSFPQQ YLHCAFNFGR KFMYQWSINW QMMDEEAFND 

       310        320        330        340        350        360 
KRFHLALLIS HLIALTTLFV TRYPRILPDL WSSLCHPLRK NAVLNANPAK TIPFVLIASN 

       370        380        390        400        410        420 
FIGVLFSRSL HYQFLSWYHW TLPILIFWSG MPFFVGPIWY VLHEWCWNSY PPNSQASTLL 

       430        440        450 
LALNTVLLLL LALTQLSGSV ALAKSHLRTT SSMEKKLN 

References and documentation are available.

Number of amino acids: 458

Molecular weight: 52860.93

Theoretical pI: 9.08

Amino acid composition: 
Ala (A)  32	  7.0%
Arg (R)  18	  3.9%
Asn (N)  16	  3.5%
Asp (D)  14	  3.1%
Cys (C)  10	  2.2%
Gln (Q)  19	  4.1%
Glu (E)  13	  2.8%
Gly (G)  16	  3.5%
His (H)  13	  2.8%
Ile (I)  28	  6.1%
Leu (L)  74	 16.2%
Lys (K)  20	  4.4%
Met (M)  21	  4.6%
Phe (F)  25	  5.5%
Pro (P)  22	  4.8%
Ser (S)  31	  6.8%
Thr (T)  19	  4.1%
Trp (W)  13	  2.8%
Tyr (Y)  22	  4.8%
Val (V)  32	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      2466
Hydrogen    H	      3810
Nitrogen    N	       606
Oxygen      O	       620
Sulfur      S	        31

Formula: C2466H3810N606O620S31
Total number of atoms: 7533

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   104905
Abs 0.1% (=1 g/l)   1.985, assuming all pairs of Cys residues form cystines


Ext. coefficient   104280
Abs 0.1% (=1 g/l)   1.973, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.48
This classifies the protein as unstable.



Aliphatic index: 114.10

Grand average of hydropathicity (GRAVY): 0.429