ProtParam
User-provided sequence:
10 20 30 40 50 60
MEGFFRIPLK RANLHGMLKA AISKIKANFT AYGAPRINIE DFNIVKEGKA EILFPKKETV
70 80 90 100 110 120
FYNPIQQFNR DLSVTCIKAW DNLYGEECGQ KRNNKKSKKK RCAETNDDSS KRQKMGNGSP
130 140 150 160 170 180
KEAVGNSNRN EPYINILEAL SATGLRAIRY AHEIPHVREV IANDLLPEAV ESIKRNVEYN
190 200 210 220 230 240
SVENIVKPNL DDANVLMYRN KATNNKFHVI DLDPYGTVTP FVDAAIQSIE EGGLMLVTCT
250 260 270 280 290 300
DLSVLAGNGY PEKCFALYGG ANMVSHESTH ESALRLVLNL LKQTAAKYKK TVEPLLSLSI
310 320 330 340 350 360
DFYVRVFVKV KTSPIEVKNV MSSTMTTYHC SRCGSYHNQP LGRISQREGR NNKTFTKYSV
370 380 390 400 410 420
AQGPPVDTKC KFCEGTYHLA GPMYAGPLHN KEFIEEVLRI NKEEHRDQDD TYGTRKRIEG
430 440 450 460 470 480
MLSLAKNELS DSPFYFSPNH IASVIKLQVP PLKKVVAGLG SLGFECSLTH AQPSSLKTNA
490 500 510 520 530 540
PWDAIWYVMQ KCDDEKKDLS KMNPNTTGYK ILSAMPGWLS GTVKSEYDSK LSFAPNEQSG
550 560 570
NIEKLRKLKI VRYQENPTKN WGPKARPNTS
References and
documentation are available.
Number of amino acids: 570
Molecular weight: 64052.24
Theoretical pI: 9.19
Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 80
Atomic composition:
Carbon C 2840
Hydrogen H 4505
Nitrogen N 791
Oxygen O 847
Sulfur S 24
Formula: C
2840H
4505N
791O
847S
24
Total number of atoms: 9007
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 62395
Abs 0.1% (=1 g/l) 0.974, assuming all pairs of Cys residues form cystines
Ext. coefficient 61770
Abs 0.1% (=1 g/l) 0.964, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 48.17
This classifies the protein as unstable.
Aliphatic index: 77.16
Grand average of hydropathicity (GRAVY): -0.536