ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEGLKKKIFG VCLKNDLAQT RNETKGIHYG LMTLETSKQL QEFLHLLVIK REVIQNFELL 

        70         80         90        100        110        120 
FHIINVAVKI TDSNLPSDDI WHFILKLRFS SEINIDEDSK VLNYLLETGI AMENPVSWKC 

       130        140        150        160        170        180 
LAVISSILSS VPQSKKIITN LIETEHAKKI GQLFDNIQDL QQGNFLVEIL SNCFKKSASN 

       190        200        210        220        230        240 
SKKVEKIPQL WQSRSKNKFF FENEFYPFSS KNGSLQTCQF LCNNFMSTLS FTGILRQVSY 

       250        260        270        280        290        300 
SGSETLKNLR IFKKKDDENS YFIQCIYNKI YLWLDEKAPL EFERKKIRIT KNLKNKIQIK 

       310        320        330        340        350        360 
LRQPFHECVR TTADKTALLF NKTKGFQLEF EDEKLGETFF HNVNNIPKIS EVQNFLVLDY 

       370        380        390        400        410        420 
IEEEPENEGE EEEQIGRADE QKEDEEEESL DELSTPMVYP IKSSIPHNHN EKVQLVTPDR 

       430        440        450        460        470        480 
SVSIRSDEWD LKSNTEDEEG NVLADLKISS TKETKRQTDY VHIDSEDQSP VVSAQMRKMR 

       490        500        510        520        530        540 
RESTKTLEIL RQEFKDKDVQ NKEDQSEQIQ NPFVNTSSLV VGKSCLVNPK EKPNIDQTVV 

       550        560        570        580        590        600 
GITELKSNSS IKKRDINILD TIFGQPPSKK QKQFHKKEKK KQQKKLTNFK PIIDVPSQDK 

       610        620        630        640        650        660 
RNLRSNAPTK PKSIKVSKLR TDKKVTGEKS SPETAAEKVD DQTVRSNDEQ AMSRATKEKC 

       670        680        690        700        710        720 
FPDVNEGKEI TKDDAKVSLE SKKNNETFVD SSVVEKHTPP DKDCNNCNIT DILESTTVID 

       730        740        750        760        770        780 
LQSPHGLSAP GQNTFTNKLQ EQIYSSINHF SNELVRKISI INQELNKKIL KELSEKYQKL 

       790        800        810        820 
FSELQDNFQN DTNEMLKFMG EIKDMMNLPE DQLVHAIRTR KFDNNKR 

References and documentation are available.

Number of amino acids: 827

Molecular weight: 95538.47

Theoretical pI: 6.78

Amino acid composition: 
Ala (A)  20	  2.4%
Arg (R)  28	  3.4%
Asn (N)  62	  7.5%
Asp (D)  50	  6.0%
Cys (C)  11	  1.3%
Gln (Q)  48	  5.8%
Glu (E)  78	  9.4%
Gly (G)  23	  2.8%
His (H)  15	  1.8%
Ile (I)  62	  7.5%
Leu (L)  71	  8.6%
Lys (K)  98	 11.9%
Met (M)  12	  1.5%
Phe (F)  40	  4.8%
Pro (P)  30	  3.6%
Ser (S)  71	  8.6%
Thr (T)  47	  5.7%
Trp (W)   5	  0.6%
Tyr (Y)  12	  1.5%
Val (V)  44	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 128
Total number of positively charged residues (Arg + Lys): 126

Atomic composition:

Carbon      C	      4215
Hydrogen    H	      6774
Nitrogen    N	      1154
Oxygen      O	      1324
Sulfur      S	        23

Formula: C4215H6774N1154O1324S23
Total number of atoms: 13490

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    46005
Abs 0.1% (=1 g/l)   0.482, assuming all pairs of Cys residues form cystines


Ext. coefficient    45380
Abs 0.1% (=1 g/l)   0.475, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.92
This classifies the protein as unstable.



Aliphatic index: 80.57

Grand average of hydropathicity (GRAVY): -0.755