ProtParam
User-provided sequence:
10 20 30 40 50 60
MEGSSSTIAR KTWELENSIL TVDSPDSTSD NIFYYDDTSQ TRFQQEKPWE NDPHYFKRVK
70 80 90 100 110 120
ISALALLKMV VHARSGGTIE IMGLMQGKTD GDTIIVMDAF ALPVEGTETR VNAQDDAYEY
130 140 150 160 170 180
MVEYSQTNKL AGRLENVVGW YHSHPGYGCW LSGIDVSTQR LNQQHQEPFL AVVIDPTRTV
190 200 210 220 230 240
SAGKVEIGAF RTYSKGYKPP DEPVSEYQTI PLNKIEDFGV HCKQYYSLDV TYFKSSLDSH
250 260 270 280 290 300
LLDLLWNKYW VNTLSSSPLL GNGDYVAGQI SDLAEKLEQA ESHLVQSRFG GVVPSSLHKK
310 320 330 340 350
KEDESQLTKI TRDSAKITVE QVHGLMSQVI KDELFNSMRQ SNNKSPTDSS DPDPMITY
References and
documentation are available.
Number of amino acids: 358
Molecular weight: 40318.08
Theoretical pI: 5.11
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1785
Hydrogen H 2763
Nitrogen N 475
Oxygen O 568
Sulfur S 11
Formula: C
1785H
2763N
475O
568S
11
Total number of atoms: 5602
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 58455
Abs 0.1% (=1 g/l) 1.450, assuming all pairs of Cys residues form cystines
Ext. coefficient 58330
Abs 0.1% (=1 g/l) 1.447, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 45.93
This classifies the protein as unstable.
Aliphatic index: 76.73
Grand average of hydropathicity (GRAVY): -0.525