ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEHQNAVKEA LNALYHHPDD TVRVHADRWL QNFQGTLDAW QVADNLLHDS SSNLETLIFC 

        70         80         90        100        110        120 
SQTLRSKVQR DFEELPPGAF QKLRQSLTTL LKKFHKGPPK VRTQISIAVA ALAVHVPAAD 

       130        140        150        160        170        180 
WGDGGIISWL RDEMHMHPEY VPGFLELLTV LPEETFNYKI AARPDRRRQF EKELTSQMEA 

       190        200        210        220        230        240 
ALSILSACLK ISELKEQVLE AFASWLRLRH GIPGTVLACH PLVHAALSSL NCDPLSEASV 

       250        260        270        280        290        300 
NVISELIHHT ASPSSGGISA QTPLIQVIVP QILSLQAHLR DSSKDEEDVK AIGRLFADVG 

       310        320        330        340        350        360 
DSYVELIATG SDEPMVIVHA LLEVTAHPEF DIASMTFNFW HSLQLMLTKR ESYSSLGSEA 

       370        380        390        400        410        420 
SIEVERNRRL HIFQPAYQSL VSLVGFRVQY PEDYQGLSYE DLKEFKQTRY AVADVLIDAA 

       430        440        450        460        470        480 
LILGGDTTLK ILYMKLLEAN AQTGNNFQDW RPAEAILFCI WAISNYVSVV EAEVMPQVMA 

       490        500        510        520        530        540 
LLQNLPQQAQ LLQTACLLVG AYSKWLNAAP ASVSILPSII RILMSGMGTS EDCAAAAALA 

       550        560        570        580        590        600 
FRHTCDDCRK NLCGYFEDLF NIYCMAINGG GGYKVSAEDS LNLVEALGMV VTELPLDQAK 

       610        620        630        640        650        660 
GALEKLCFSA ASPLEEAAKE DLEKKHAREL TVHIDRFAFL FRYVNHPEAV AAEINKHWAI 

       670        680        690        700        710        720 
FRVIFDARPW DMRTMESLCR ACKYAVRTSG RYIIDTIGEM LEKIQFHYQQ HHQPCFLYLS 

       730        740        750        760        770        780 
SEVIKIFGSD PSCAVYLKNL IETLFAHTTC LMTSIKEVTA RPDIADDCFL LASRCLRYCP 

       790        800        810        820        830        840 
HLFIPSPIFP ALVNCAMIGI TVQHREACHS ILTFLTDIFD LEKSVNEEQF VRIRDNIIIP 

       850        860        870        880        890        900 
RGATITRILI ASLAGALPSS RLDTVTYSLL ALTRTYRLQA VSWAKESVSL IPRTALTETE 

       910        920        930        940        950 
STKFLQALSD IAYGADVNSL IGQVEELSDV CRRNRTVQEL VQAALKPLEL NLVTAPVS 

References and documentation are available.

Number of amino acids: 958

Molecular weight: 106917.02

Theoretical pI: 5.62

Amino acid composition: 
Ala (A)  98	 10.2%
Arg (R)  49	  5.1%
Asn (N)  29	  3.0%
Asp (D)  47	  4.9%
Cys (C)  23	  2.4%
Gln (Q)  42	  4.4%
Glu (E)  65	  6.8%
Gly (G)  38	  4.0%
His (H)  31	  3.2%
Ile (I)  62	  6.5%
Leu (L) 118	 12.3%
Lys (K)  36	  3.8%
Met (M)  19	  2.0%
Phe (F)  38	  4.0%
Pro (P)  41	  4.3%
Ser (S)  72	  7.5%
Thr (T)  49	  5.1%
Trp (W)  12	  1.3%
Tyr (Y)  26	  2.7%
Val (V)  63	  6.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 112
Total number of positively charged residues (Arg + Lys): 85

Atomic composition:

Carbon      C	      4789
Hydrogen    H	      7562
Nitrogen    N	      1286
Oxygen      O	      1401
Sulfur      S	        42

Formula: C4789H7562N1286O1401S42
Total number of atoms: 15080

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   106115
Abs 0.1% (=1 g/l)   0.992, assuming all pairs of Cys residues form cystines


Ext. coefficient   104740
Abs 0.1% (=1 g/l)   0.980, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.44
This classifies the protein as unstable.



Aliphatic index: 102.58

Grand average of hydropathicity (GRAVY): 0.053