ProtParam
User-provided sequence:
10 20 30 40 50 60
MEISGKMHEG DSLVVSPKSL DELPINVIFQ ILSELKFSDL KNVSATCWTL RILTNEKLMY
70 80 90 100 110 120
YSILHDPKNQ YLWTKRYFLD SLRMLQRNRR GFDFVSLNDT TFFQSLRYLH NGVRKVSGNL
130 140 150 160 170 180
LRLLESNDDL GINEIDTASE SEDNDNMNND ENDNESNGYD SQNQRVGKKY INDGRTDGEM
190 200 210 220 230 240
MPDNSDLWET EDESDIDTTN DVNGLDQIYA ENISVFEEDT GSDSYNDGQI QPVQISMNDF
250 260 270 280 290 300
ADSESTPTKK PRTKPTKIDK EGLKYLRVLE GFHRIGVISG RETPDRIITH NTSATPNKYG
310 320 330 340 350 360
NLLDDVQFTP LSTISSLLKR IDSGSDSNHA HSPDSFGISH SRSGSSVFSD GAPKLADQPW
370 380 390 400 410 420
SKIYELDHQS LSSSDSDSSS STEFIRQLQS SKKVKDKAVL FERLLAKSKE RSVSLKEKKS
430 440 450 460 470 480
HASSNNGNCR KVSDDYLAET RRNASPAPSS VSSIDTEMRL TMQEVKNNSN DHFDSHAKTK
490 500 510
SSSTHGIKHS KIHHRKKLKA LVMDGNRICY EKITE
References and
documentation are available.
Number of amino acids: 515
Molecular weight: 58166.26
Theoretical pI: 5.65
Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 66
Atomic composition:
Carbon C 2505
Hydrogen H 3970
Nitrogen N 724
Oxygen O 843
Sulfur S 14
Formula: C
2505H
3970N
724O
843S
14
Total number of atoms: 8056
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 42985
Abs 0.1% (=1 g/l) 0.739, assuming all pairs of Cys residues form cystines
Ext. coefficient 42860
Abs 0.1% (=1 g/l) 0.737, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.98
This classifies the protein as unstable.
Aliphatic index: 70.78
Grand average of hydropathicity (GRAVY): -0.840