ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEISSSPWND GGYSPYERNR VAVSPFSSAL EGEERIETSR SLGDHCFEPL PYVTNYLSIF 

        70         80         90        100        110        120 
ALFGKEIFGD KGNVSSRNEY LLKKYYSLKK PFVLRHNGHA LKNPDMPLQR NDILQTNFMV 

       130        140        150        160        170        180 
DKFLNRTVRS VNFNNFKIIS DMQSKSGRGT KSGTNQNQSA DAIQNICLPS IPSALPYFQY 

       190        200        210        220        230        240 
YRKLLTVNTK EWDILKLHSL WVPKLRKDFK DFSLYGDKNS LKPIDSHYDE DNTMKKNLFF 

       250        260        270        280        290        300 
ERSPSRQTLD GKGCASKGYD ISSGNMIIPS LFSEDKLPAL TYHCSVELNG NIYIFGGLMP 

       310        320        330        340        350        360 
CYSYEEDAPM LNDFFVDGIK NLPPPLLPQV INNPSMVNNP HLYVASIPSC RFSKPKMGGY 

       370        380        390        400        410        420 
IPPPLLCVQG SKLTDRHIFF YGGFEIRTET RGDENGKYHL KKRLYVNNTG YILDIMSFKF 

       430        440        450        460        470        480 
TKIDIIVQPS KYNAYPTMSS RFGHLQISID NPNRRASVHS SSMNEIHKMG SASMKQGSSI 

       490        500        510        520        530        540 
TSGRLEKAAV LSSLPHNTVH TVIIFGGYRQ TGDDRYEAMN DLWKIEIPVI RRGKKGYCKF 

       550        560        570        580        590        600 
SETANAILLT PSEKDKSDWP EERAFSAFSV HGTSLMDRSS LDMRLLNNLK NHFVLKPSYI 

       610        620        630        640        650        660 
SQDRVVSPKP VFPMMVHGTH QDLFNSGSAA QESPKAGASA SSASAASFDP DMDDNLENYI 

       670        680        690        700        710        720 
INPGRKSSSI PMTAIGRQRL ILSQEKPVGK TVVLHGGSNG LNVLDDMWLM DLECETWTPI 

       730        740        750        760        770        780 
ETFAKADSSE DGDEKLDSVN VGLVGHRMES IGRICVCIGG MVQEDVDQFY SENDDEPPRK 

       790        800        810        820        830        840 
RKVDTLPLGG NFLNTIDLST QFWEEHKITL SKKAADEDRQ DSENEDTNSN IVVGVGGTSF 


NVTKVLF 

References and documentation are available.

Number of amino acids: 847

Molecular weight: 95108.68

Theoretical pI: 6.74

Amino acid composition: 
Ala (A)  34	  4.0%
Arg (R)  39	  4.6%
Asn (N)  56	  6.6%
Asp (D)  56	  6.6%
Cys (C)  11	  1.3%
Gln (Q)  23	  2.7%
Glu (E)  44	  5.2%
Gly (G)  58	  6.8%
His (H)  21	  2.5%
Ile (I)  51	  6.0%
Leu (L)  71	  8.4%
Lys (K)  58	  6.8%
Met (M)  26	  3.1%
Phe (F)  40	  4.7%
Pro (P)  49	  5.8%
Ser (S)  89	 10.5%
Thr (T)  37	  4.4%
Trp (W)   8	  0.9%
Tyr (Y)  31	  3.7%
Val (V)  45	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 100
Total number of positively charged residues (Arg + Lys): 97

Atomic composition:

Carbon      C	      4216
Hydrogen    H	      6567
Nitrogen    N	      1151
Oxygen      O	      1284
Sulfur      S	        37

Formula: C4216H6567N1151O1284S37
Total number of atoms: 13255

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    90815
Abs 0.1% (=1 g/l)   0.955, assuming all pairs of Cys residues form cystines


Ext. coefficient    90190
Abs 0.1% (=1 g/l)   0.948, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.78
This classifies the protein as unstable.



Aliphatic index: 75.60

Grand average of hydropathicity (GRAVY): -0.476