ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEIVYKPLDI RNEEQFASIK KLIDADLSEP YSIYVYRYFL NQWPELTYIA VDNKSGTPNI 

        70         80         90        100        110        120 
PIGCIVCKMD PHRNVRLRGY IGMLAVESTY RGHGIAKKLV EIAIDKMQRE HCDEIMLETE 

       130        140        150        160        170 
VENSAALNLY EGMGFIRMKR MFRYYLNEGD AFKLILPLTE KSCTRSTFLM HGRLAT 

References and documentation are available.

Number of amino acids: 176

Molecular weight: 20456.82

Theoretical pI: 6.90

Amino acid composition: 
Ala (A)  10	  5.7%
Arg (R)  12	  6.8%
Asn (N)   8	  4.5%
Asp (D)   8	  4.5%
Cys (C)   4	  2.3%
Gln (Q)   3	  1.7%
Glu (E)  15	  8.5%
Gly (G)  10	  5.7%
His (H)   4	  2.3%
Ile (I)  16	  9.1%
Leu (L)  17	  9.7%
Lys (K)  11	  6.2%
Met (M)   9	  5.1%
Phe (F)   6	  3.4%
Pro (P)   7	  4.0%
Ser (S)   8	  4.5%
Thr (T)   8	  4.5%
Trp (W)   1	  0.6%
Tyr (Y)  11	  6.2%
Val (V)   8	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       916
Hydrogen    H	      1447
Nitrogen    N	       243
Oxygen      O	       261
Sulfur      S	        13

Formula: C916H1447N243O261S13
Total number of atoms: 2880

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    22140
Abs 0.1% (=1 g/l)   1.082, assuming all pairs of Cys residues form cystines


Ext. coefficient    21890
Abs 0.1% (=1 g/l)   1.070, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.14
This classifies the protein as stable.



Aliphatic index: 91.99

Grand average of hydropathicity (GRAVY): -0.222