ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKDLSSLYS EKKDKENDET LFNIKLSKSV VETTPLNGHS LFDDDKSLSD WTDNVFTQSV 

        70         80         90        100        110        120 
FYHGSDDLIW GKFFVCVYKS PNSNKLNAII FDKLGTSCFE SVDISSNSQY YPAIENLSPS 

       130        140        150        160        170        180 
DQESNVKKCI AVILLQRYPL LSPSDLSQIL SNKSENCDYD PPYAGDLASS CQLITAVPPE 

       190        200        210        220        230        240 
DLGKRFFTSG LLQNRFVSST LLDVIYENNE STIELNNRLV FHLGEQLEQL FNPVTEYSPE 

       250        260        270        280        290        300 
QTEYGYKAPE DELPTESDDD LVKAICNELL QLQTNFTFNL VEFLQKFLIA LRVRVLNEEI 

       310        320        330        340        350        360 
NGLSTTKLNR LFPPTIDEVT RINCIFLDSL KTAIPYGSLE VLKACSITIP YFYKAYTRHE 

       370        380        390        400        410        420 
AATKNFSKDI KLFIRHFSNV IPEREVYTEM KIESIIKGPQ EKLLKLKLII ERLWKSKKWR 

       430        440        450        460        470        480 
PKNQEMAKKC YNNIIDVIDS FGKLDSPLHS YSTRVFTPSG KILTELAKCW PVELQYKWLK 

       490        500        510        520        530        540 
RRVVGVYDVV DLNDENKRNL LVIFSDYVVF INILEAESYY TSDGSNRPLI SDILMNSLIN 

       550        560        570        580        590        600 
EVPLPSKIPK LKVERHCYID EVLVSILDKS TLRFDRLKGK DSFSMVCKLS SAFISSSSVA 

       610        620        630        640        650        660 
DLITKARILE KDTAFHLFKA SRSHFTLYST AHELCAYDSE KIKSKFALFL NIPPSKEILE 

       670        680        690        700        710        720 
VNNLHLAFFA RFCSNDGRDN IVILDVLTKH DDKHIEVTSD NIVFTIINQL AIEIPICFSS 

       730        740        750        760        770        780 
LNSSMAKDLL CVNENLIKNL EHQLEEVKHP STDEHRAVNS KLSGASDFDA THEKKRSYGT 

       790        800        810        820        830        840 
ITTFRSYTSD LKDSPSGDNS NVTKETKEIL PVKPTKKSSK KPREIQKKTK TNASKAEHIE 

       850        860        870        880        890        900 
KKKPNKGKGF FGVLKNVFGS KSKSKPSPVQ RVPKKISQRH PKSPVKKPMT SEKKSSPKRA 

       910        920        930        940        950        960 
VVSSPKIKKK STSFSTKESQ TAKSSLRAVE FKSDDLIGKP PDVGNGAHPQ ENTRISSVVR 

       970        980        990       1000       1010       1020 
DTKYVSYNPS QPVTENTSNE KNVEPKADQS TKQDNISNFA DVEVSASSYP EKLDAETDDQ 

      1030       1040       1050       1060       1070       1080 
IIGKATNSSS VHGNKELPDL AEVTTANRVS TTSAGDQRID TQSEFLRAAD VENLSDDDEH 

      1090       1100       1110       1120       1130       1140 
RQNESRVFND DLFGDFIPKH YRNKQENINS SSNLFPEGKV PQEKGVSNEN TNISLKTNED 

      1150       1160       1170       1180       1190       1200 
ASTLTQKLSP QASKVLTENS NELKDTNNEG KDAKDIKLGD DYSDKETAKE ITKPKNFVEG 

      1210       1220       1230       1240       1250       1260 
ITERKEIFPT IPRLAPPASK INFQRSPSYI ELFQGMRVVL DKHDAHYNWK RLASQVSLSE 

      1270       1280       1290       1300       1310       1320 
GLKVNTEEDA AIINKSQDDA KAERMTQISE VIEYEMQQPI PTYLPKAHLD DSGIEKSDDK 

      1330       1340       1350       1360       1370       1380 
FFEIEEELKE ELKGSKTGNE DVGNNNPSNS IPKIEKPPAF KVIRTSPVRI IGRTFEDTRK 

      1390       1400       1410       1420       1430       1440 
YENGSPSDIS FTYDTHNNDE PDKRLMELKF PSQDEIPDDR FYTPAEEPTA EFPVEELPNT 

      1450       1460       1470       1480       1490       1500 
PRSINVTTSN NKSTDDKLSS GNIDQKPTEL LDDLEFSSFN IAFGNTSMST DNMKISSDLS 

      1510       1520       1530       1540       1550       1560 
SNKTVLGNAQ KVQESPSGPL IYVLPQSSTK HEKEGFLRKK QKDEPIWVSP SKIDFADLSR 

      1570       1580       1590       1600       1610       1620 
RTKALTPERN TVPLKNNDSR KYKYTGEGSI GNMTNMLLTK DASYAYLKDF VALSDDEDED 

      1630 
GKQNCAVGGP EKLKFY 

References and documentation are available.

Number of amino acids: 1636

Molecular weight: 184718.86

Theoretical pI: 5.84

Amino acid composition: 
Ala (A)  72	  4.4%
Arg (R)  59	  3.6%
Asn (N) 109	  6.7%
Asp (D) 117	  7.2%
Cys (C)  17	  1.0%
Gln (Q)  48	  2.9%
Glu (E) 128	  7.8%
Gly (G)  56	  3.4%
His (H)  28	  1.7%
Ile (I) 102	  6.2%
Leu (L) 140	  8.6%
Lys (K) 163	 10.0%
Met (M)  15	  0.9%
Phe (F)  72	  4.4%
Pro (P)  89	  5.4%
Ser (S) 176	 10.8%
Thr (T)  99	  6.1%
Trp (W)   8	  0.5%
Tyr (Y)  47	  2.9%
Val (V)  91	  5.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 245
Total number of positively charged residues (Arg + Lys): 222

Atomic composition:

Carbon      C	      8173
Hydrogen    H	     12957
Nitrogen    N	      2197
Oxygen      O	      2606
Sulfur      S	        32

Formula: C8173H12957N2197O2606S32
Total number of atoms: 25965

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   115030
Abs 0.1% (=1 g/l)   0.623, assuming all pairs of Cys residues form cystines


Ext. coefficient   114030
Abs 0.1% (=1 g/l)   0.617, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.20
This classifies the protein as unstable.



Aliphatic index: 78.22

Grand average of hydropathicity (GRAVY): -0.651