ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKFGLKALV PLLKLEDKEL SSTYDHSMTL GADLSSMLYS LGIPRDSQDH RVLDTFQSPW 

        70         80         90        100        110        120 
AETSRSEVEP RFFTPESFTN IPGVLQSTVT PPCFNSIQND QQRVALFQDE TLFFLFYKHP 

       130        140        150        160        170        180 
GTVIQELTYL ELRKRNWRYH KTLKAWLTKD PMMEPIVSAD GLSERGSYVF FDPQRWEKCQ 

       190 
RDFLLFYNAI M 

References and documentation are available.

Number of amino acids: 191

Molecular weight: 22362.51

Theoretical pI: 5.60

Amino acid composition: 
Ala (A)   7	  3.7%
Arg (R)  11	  5.8%
Asn (N)   5	  2.6%
Asp (D)  12	  6.3%
Cys (C)   2	  1.0%
Gln (Q)  10	  5.2%
Glu (E)  13	  6.8%
Gly (G)   7	  3.7%
His (H)   4	  2.1%
Ile (I)   6	  3.1%
Leu (L)  23	 12.0%
Lys (K)  10	  5.2%
Met (M)   6	  3.1%
Phe (F)  14	  7.3%
Pro (P)  12	  6.3%
Ser (S)  16	  8.4%
Thr (T)  13	  6.8%
Trp (W)   4	  2.1%
Tyr (Y)   7	  3.7%
Val (V)   9	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1016
Hydrogen    H	      1547
Nitrogen    N	       261
Oxygen      O	       293
Sulfur      S	         8

Formula: C1016H1547N261O293S8
Total number of atoms: 3125

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    32555
Abs 0.1% (=1 g/l)   1.456, assuming all pairs of Cys residues form cystines


Ext. coefficient    32430
Abs 0.1% (=1 g/l)   1.450, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.71
This classifies the protein as unstable.



Aliphatic index: 76.54

Grand average of hydropathicity (GRAVY): -0.406