ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MEKHQYRDLQ LIGQGSFGSV FRAQDVESSK IVALKVVDLD ATKDQIETLT QEINFLIDLN 

        70         80         90        100        110        120 
SVHITKYYAS FVDGFRLWIT MEYCDGGSCL DLLKLSGTFS ERVIAEVMRQ VLEALVYLHG 

       130        140        150        160        170        180 
QGKMHRDIKA ANILTMKDGL VKLADFGVSG QLESLRDKND DFVGTPFWMA PEVVKQTGYN 

       190        200        210        220        230        240 
YKADIWSLGI TAYELATGEP PYSGIHPMKV LLLIPKHSPP SLERSKFSRA FCDFVSNCLK 

       250        260        270        280        290        300 
KNPKDRATAE YLSKHKFIKK YCPNTSVKEV VASYAKWKES ELLPEATPYN STMGNSANTI 

       310 
DEVVWDFGTV RR 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 35218.40

Theoretical pI: 6.67

Amino acid composition: 
Ala (A)  20	  6.4%
Arg (R)  12	  3.8%
Asn (N)  11	  3.5%
Asp (D)  20	  6.4%
Cys (C)   5	  1.6%
Gln (Q)  10	  3.2%
Glu (E)  19	  6.1%
Gly (G)  19	  6.1%
His (H)   7	  2.2%
Ile (I)  16	  5.1%
Leu (L)  30	  9.6%
Lys (K)  26	  8.3%
Met (M)   8	  2.6%
Phe (F)  14	  4.5%
Pro (P)  12	  3.8%
Ser (S)  24	  7.7%
Thr (T)  17	  5.4%
Trp (W)   5	  1.6%
Tyr (Y)  13	  4.2%
Val (V)  24	  7.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1586
Hydrogen    H	      2478
Nitrogen    N	       414
Oxygen      O	       466
Sulfur      S	        13

Formula: C1586H2478N414O466S13
Total number of atoms: 4957

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47120
Abs 0.1% (=1 g/l)   1.338, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   1.331, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.44
This classifies the protein as stable.



Aliphatic index: 86.22

Grand average of hydropathicity (GRAVY): -0.248